dc.creator | Vila Spinola, Marta | es |
dc.creator | Díaz Santos, Encarnación | es |
dc.creator | Vega Naranjo, Marta de La | es |
dc.creator | Rodríguez Martínez, Herminia | es |
dc.creator | Vargas Muñoz, María de los Ángeles | es |
dc.creator | León, Rosa | es |
dc.date.accessioned | 2018-08-06T10:36:32Z | |
dc.date.available | 2018-08-06T10:36:32Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Vila Spinola, M., Díaz Santos, E., Vega Naranjo, M.d.L., Rodríguez Martínez, H., Vargas Muñoz, M.d.l.Á. y León, R. (2012). Promoter trapping in microalgae using the antibiotic paromomycin as selective agent. Marine Drugs, 10, 2749-2765. | |
dc.identifier.issn | 1660-3397 | es |
dc.identifier.uri | https://hdl.handle.net/11441/77864 | |
dc.description.abstract | The lack of highly active endogenous promoters to drive the expression of transgenes is one of the main drawbacks to achieving efficient transformation of many microalgal species. Using the model chlorophyte Chlamydomonas reinhardtii and the paromomycin resistance APHVIII gene from Streptomyces rimosus as a marker, we have demonstrated that random insertion of the promoterless marker gene and subsequent isolation of the most robust transformants allows for the identification of novel strong promoter sequences in microalgae. Digestion of the genomic DNA with an enzyme that has a unique restriction site inside the marker gene and a high number of target sites in the genome of the microalga, followed by inverse PCR, allows for easy determination of the genomic region, which precedes the APHVIII marker gene. In most of the transformants analyzed, the marker gene is inserted in intragenic regions and its expression relies on its adequate insertion in frame with native genes. As an example, one of the new promoters identified was used to direct the expression of the APHVIII marker gene in C. reinhardtii, showing high transformation efficiencies. | es |
dc.description.sponsorship | Junta de Andalucía (P09-CVI-5053) | es |
dc.description.sponsorship | Junta de Andalucía (BIO-214) | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Marine Drugs, 10, 2749-2765. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | APHVIII | es |
dc.subject | Chlamydomonas reinhardtii | es |
dc.subject | Microalgae transformation | es |
dc.subject | Paromomycin | es |
dc.subject | Promoter trapping | es |
dc.title | Promoter trapping in microalgae using the antibiotic paromomycin as selective agent | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | P09-CVI-5053 | es |
dc.relation.projectID | BIO-214 | es |
dc.relation.publisherversion | http://www.mdpi.com/1660-3397/10/12/2749/htm | es |
dc.identifier.doi | 10.3390/md10122749 | es |
idus.format.extent | 17 | es |
dc.journaltitle | Marine Drugs | es |
dc.publication.volumen | 10 | es |
dc.publication.initialPage | 2749 | es |
dc.publication.endPage | 2765 | es |
dc.contributor.funder | Junta de Andalucía | |
dc.contributor.funder | Junta de Andalucía | |