Autor: |
Makhdoumi Kakhki, Ali
Amoozegar, Mohammad Ali Kazemi, Bahram Ventosa Ucero, Antonio |
Departamento: | Universidad de Sevilla. Departamento de Microbiología y Parasitología |
Fecha: | 2017 |
Publicado en: | Microbes and Environments, 27 (1), 87-93. |
Tipo de documento: | Artículo |
Resumen: |
Prokaryotic diversity in Aran-Bidgol salt lake, a thalasohaline lake in Iran, was studied by fluorescence in situ hybridization (FISH), cultivation techniques, denaturing gradient gel electrophoresis (DGGE) of PCR-amplified fragments of 16S rRNA genes and 16S rRNA gene clone library analysis. Viable counts obtained (2.5–4 × 106 cells mL−1) were similar to total cell abundance in the lake determined by DAPI direct count (3–4×107 cells mL−1). The proportion of Bacteria to Archaea in the community detectable by FISH was unexpectedly high and ranged between 1:3 and 1:2. We analyzed 101 archaeal isolates and found that most belonged to the genera Halorubrum (55%) and Haloarcula (18%). Eleven bacterial isolates obtained in pure culture were affiliated with the genera Salinibacter (18.7%), Salicola (18.7%) and Rhodovibrio (35.3%). Analysis of inserts of 100 clones from the eight 16S rRNA clone libraries constructed revealed 37 OTUs. The majority (63%) of these sequences were not related to a... [Ver más] |
Cita: | Makhdoumi Kakhki, A., Amoozegar, M.A., Kazemi, . y Ventosa Ucero, A. (2017). Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran. Microbes and Environments, 27 (1), 87-93. |
URI: https://hdl.handle.net/11441/75683
DOI: 10.1264/jsme2.ME11267
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