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Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran

Opened Access Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran

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Autor: Makhdoumi Kakhki, Ali
Amoozegar, Mohammad Ali
Kazemi, Bahram
Ventosa Ucero, Antonio
Departamento: Universidad de Sevilla. Departamento de Microbiología y Parasitología
Fecha: 2017
Publicado en: Microbes and Environments, 27 (1), 87-93.
Tipo de documento: Artículo
Resumen: Prokaryotic diversity in Aran-Bidgol salt lake, a thalasohaline lake in Iran, was studied by fluorescence in situ hybridization (FISH), cultivation techniques, denaturing gradient gel electrophoresis (DGGE) of PCR-amplified fragments of 16S rRNA genes and 16S rRNA gene clone library analysis. Viable counts obtained (2.5–4 × 106 cells mL−1) were similar to total cell abundance in the lake determined by DAPI direct count (3–4×107 cells mL−1). The proportion of Bacteria to Archaea in the community detectable by FISH was unexpectedly high and ranged between 1:3 and 1:2. We analyzed 101 archaeal isolates and found that most belonged to the genera Halorubrum (55%) and Haloarcula (18%). Eleven bacterial isolates obtained in pure culture were affiliated with the genera Salinibacter (18.7%), Salicola (18.7%) and Rhodovibrio (35.3%). Analysis of inserts of 100 clones from the eight 16S rRNA clone libraries constructed revealed 37 OTUs. The majority (63%) of these sequences were not related to a...
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Cita: Makhdoumi Kakhki, A., Amoozegar, M.A., Kazemi, . y Ventosa Ucero, A. (2017). Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran. Microbes and Environments, 27 (1), 87-93.
Tamaño: 950.0Kb
Formato: PDF

URI: https://hdl.handle.net/11441/75683

DOI: 10.1264/jsme2.ME11267

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