dc.creator | Makhdoumi Kakhki, Ali | es |
dc.creator | Amoozegar, Mohammad Ali | es |
dc.creator | Kazemi, Bahram | es |
dc.creator | Ventosa Ucero, Antonio | es |
dc.date.accessioned | 2018-06-05T11:30:21Z | |
dc.date.available | 2018-06-05T11:30:21Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Makhdoumi Kakhki, A., Amoozegar, M.A., Kazemi, . y Ventosa Ucero, A. (2017). Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran. Microbes and Environments, 27 (1), 87-93. | |
dc.identifier.issn | 1347-4405 | es |
dc.identifier.uri | https://hdl.handle.net/11441/75683 | |
dc.description.abstract | Prokaryotic diversity in Aran-Bidgol salt lake, a thalasohaline lake in Iran, was studied by fluorescence in situ hybridization (FISH), cultivation techniques, denaturing gradient gel electrophoresis (DGGE) of PCR-amplified fragments of 16S rRNA genes and 16S rRNA gene clone library analysis. Viable counts obtained (2.5–4 × 106 cells mL−1) were similar to total cell abundance in the lake determined by DAPI direct count (3–4×107 cells mL−1). The proportion of Bacteria to Archaea in the community detectable by FISH was unexpectedly high and ranged between 1:3 and 1:2. We analyzed 101 archaeal isolates and found that most belonged to the genera Halorubrum (55%) and Haloarcula (18%). Eleven bacterial isolates obtained in pure culture were affiliated with the genera Salinibacter (18.7%), Salicola (18.7%) and Rhodovibrio (35.3%). Analysis of inserts of 100 clones from the eight 16S rRNA clone libraries constructed revealed 37 OTUs. The majority (63%) of these sequences were not related to any previously identified taxa. Within this sampling effort we most frequently retrieved phylotypes related to Halorhabdus (16% of archaeal sequences obtained) and Salinibacter (36% of bacterial sequences obtained). Other prokaryotic groups that were abundant included representatives of Haloquadratum, the anaerobic genera Halanaerobium and Halocella, purple sulfur bacteria of the genus Halorhodospira and Cyanobacteria. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | The Japanese Society of Microbial Ecology | es |
dc.relation.ispartof | Microbes and Environments, 27 (1), 87-93. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Archaea | es |
dc.subject | Bacteria | es |
dc.subject | halophilic microorganisms | es |
dc.subject | prokaryotic diversity | es |
dc.subject | hypersaline lake | es |
dc.title | Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología y Parasitología | es |
dc.relation.publisherversion | http://dx.doi.org10.1264/jsme2.ME11267 | es |
dc.identifier.doi | 10.1264/jsme2.ME11267 | es |
idus.format.extent | 6 p. | es |
dc.journaltitle | Microbes and Environments | es |
dc.publication.volumen | 27 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 87 | es |
dc.publication.endPage | 93 | es |