Repositorio de producción científica de la Universidad de Sevilla

Synthesis of amine-functionalized heparin oligosaccharides for the investigation of carbohydrate-protein interactions in microtiter plates

Opened Access Synthesis of amine-functionalized heparin oligosaccharides for the investigation of carbohydrate-protein interactions in microtiter plates

Citas

buscar en

Estadísticas
Icon
Exportar a
Autor: Maza Pérez, Susana
Macchione, Giuseppe
Ojeda, Rafael
López Prados, Javier
Angulo, Jesús
Paz, José L. de
Nieto, Pedro M.
Fecha: 2012
Publicado en: Organic and Biomolecular Chemistry, 10, 2146-2163.
Tipo de documento: Artículo
Resumen: The synthesis of well-defined oligosaccharides is crucial for the establishment of structure-activity relationships for specific sequences of heparin, contributing to the understanding of the biological role of this polysaccharide. It is highly convenient that the synthetic oligosaccharides contain an orthogonal functional group that allows selective conjugation of the probes and expands their use as chemical tools in glycobiology. We present here the synthesis of a series of amine-functionalized heparin oligosaccharides using a n+2 modular approach. The conditions of the glycosylation reactions were carefully optimized to produce efficiently the desired synthetic intermediates with an N-benzyloxycarbonyl-protected aminoethyl spacer at the reducing end. The use of microwave heating greatly facilitates O- and N-sulfation steps, avoiding experimental problems associated with these reactions. The synthesized oligosaccharides were immobilized in 384-well microtiter plates and successfully...
[Ver más]
Cita: Maza Pérez, S., Macchione, G., Ojeda, R., López Prados, J., Angulo, J., Paz, J.L.d. y Nieto, P.M. (2012). Synthesis of amine-functionalized heparin oligosaccharides for the investigation of carbohydrate-protein interactions in microtiter plates. Organic and Biomolecular Chemistry, 10, 2146-2163.
Tamaño: 1.005Mb
Formato: PDF

URI: https://hdl.handle.net/11441/70867

DOI: 10.1039/C2OB06607F

Ver versión del editor

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones