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Tension in secretory granule membranes causes extensive membrane transfer through the exocytotic fusion pore

Opened Access Tension in secretory granule membranes causes extensive membrane transfer through the exocytotic fusion pore

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Autor: Monck, Jonathan R.
Álvarez de Toledo Naranjo, Guillermo
Fernández, Julio M.
Departamento: Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica
Fecha: 1990
Publicado en: Proceedings of the National Academy of Sciences of the United States of America, 87 (20), 7804-7808.
Tipo de documento: Artículo
Resumen: or fusion to occur the repulsive forces between two interacting phospholipid bilayers must be reduced. In model systems, this can be achieved by increasing the surface tension of at least one of the membranes. However, there has so far been no evidence that the secretory granule membrane is under tension. We have been studying exocytosis by using the patch-clamp technique to measure the surface area of the plasma membrane of degranulating mast cells. When a secretory granule fuses with the plasma membrane there is a step increase in the cell surface area. Some fusion events are reversible, in which case we have found that the backstep is larger than the initial step, indicating that there is a net decrease in the area of the plasma membrane. The decrease has the following properties: (i) the magnitude is strongly dependent on the lifetime of the fusion event and can be extensive, representing as much as 40% of the initial granule surface area; (ii) the rate of decrease is independent ...
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Cita: Monck, J.R., Álvarez de Toledo Naranjo, G. y Fernández, J.M. (1990). Tension in secretory granule membranes causes extensive membrane transfer through the exocytotic fusion pore. Proceedings of the National Academy of Sciences of the United States of America, 87 (20), 7804-7808.
Tamaño: 1.073Mb
Formato: PDF

URI: https://hdl.handle.net/11441/69939

DOI: 10.1073/pnas.87.20.7804

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