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Glutathione biosynthesis in Arabidopsis trichome cells

Opened Access Glutathione biosynthesis in Arabidopsis trichome cells

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Autor: Gutiérrez Alcalá, Gloria
Gotor Martínez, Cecilia
Meyer, Andreas J.
Fricker, Mark
Vega, Jorge M.
Romero González, Luis Carlos
Departamento: Universidad de Sevilla. Departamento de Química Inorgánica
Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2000
Publicado en: Proceedings of the National Academy of Sciences of the United States of America, 97 (20), 11108-11113.
Tipo de documento: Artículo
Resumen: In Arabidopsis thaliana, trichome cells are specialized unicellular structures with uncertain functions. Based on earlier observations that one of the genes involved in cysteine biosynthesis (Atcys-3A) is highly expressed in trichomes, we have extended our studies in trichome cells to determine their capacity for glutathione (GSH) biosynthesis. First, we have analyzed by in situ hybridization the tissue-specific expression of the genes Atcys-3A and sat5, which encode O-acetylserine(thio)lyase (OASTL) and serine acetyltransferase (SAT), respectively, as well as gsh1 and gsh2, which encode γ-glutamylcysteine synthetase and glutathione synthetase, respectively. The four genes are highly expressed in leaf trichomes of Arabidopsis, and their mRNA accumulate to high levels. Second, we have directly measured cytoplasmic GSH concentration in intact cells by laser-scanning microscopy after labeling with monochlorobimane as a GSH-specific probe. From these measurements, cytosolic GSH concentrat...
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Cita: Gutiérrez-Alcalá, G., Gotor, C., Meyer, A.J., Fricker, M., Vega, J.M. y Romero, L.C. (2000). Glutathione biosynthesis in Arabidopsis trichome cells. Proceedings of the National Academy of Sciences of the United States of America, 97 (20), 11108-11113.
Tamaño: 709.1Kb
Formato: PDF

URI: https://hdl.handle.net/11441/69627

DOI: 10.1073/pnas.190334497

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