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A peripheral protein associated with the cis-Golgi network redistributes in the intermediate compartment upon brefeldin A treatment

Opened Access A peripheral protein associated with the cis-Golgi network redistributes in the intermediate compartment upon brefeldin A treatment

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Autor: Ríos Sánchez, Rosa María
Tassin, Anne-Marie
Celati, Claude
Antony, Claude
Boissier, Marie-Christophe
Homberg, Jean-Claude
Bornens, Michel
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 1994
Publicado en: Journal of Cell Biology, 125 (5), 997-1013.
Tipo de documento: Artículo
Resumen: Human autoantibodies offer unique tools for the study of cellular constituents since they usually recognize highly conserved components, the most difficult to detect due to their low immunogenicity. The serum from a patient with Sjogren's syndrome (RM serum) showing a very high reactivity to the Golgi complex has been shown to immunoprecipitate and to immunodetect by Western blotting experiments a protein of mol wt 210,000 (p210) that was shown to be peripheral and cytoplasmically disposed. A close examination of the p210 labeling revealed some differences with Golgi markers: RM serum staining was slightly more extensive than several Golgi markers and showed a discontinuous or granular appearance. Nocodazole induced a specific and early segregation of many p210-associated vesicles or tubules from Golgi apparatus. Upon brefeldin A treatment, p210 did not redistribute in the ER as did other Golgi proteins. In contrast, it exhibited a vesicular pattern reminiscent to that displayed by pr...
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Cita: Ríos Sánchez, R.M., Tassin, A., Celati, C., Antony, C., Boissier, M., Homberg, J. y Bornens, M. (1994). A peripheral protein associated with the cis-Golgi network redistributes in the intermediate compartment upon brefeldin A treatment. Journal of Cell Biology, 125 (5), 997-1013.
Tamaño: 6.605Mb
Formato: PDF

URI: http://hdl.handle.net/11441/65012

DOI: 10.1083/jcb.125.5.997

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