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Use of real-time PCR and fluorimetry for rapid detection of rifampin and isoniazid resistance-associated mutations in Mycobacterium tuberculosis

 

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Opened Access Use of real-time PCR and fluorimetry for rapid detection of rifampin and isoniazid resistance-associated mutations in Mycobacterium tuberculosis
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Author: Torres Sánchez, María José
Criado, Antonio
Palomares Folía, José Carlos
Aznar Martín, Javier
Department: Universidad de Sevilla. Departamento de Microbiología
Date: 2000
Published in: Journal of Clinical Microbiology, 38 (9), 3194-3199.
Document type: Article
Abstract: Very fast amplification of DNA in small volumes can be continuously monitored with a rapid cycler that incorporates fluorimetric detection. Primers were designed to amplify a 157-bp fragment of the rpoB gene spanning codons 526 and 531 and a 209-bp ...
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Cite: Torres Sánchez, M.J., Criado, A., Palomares Folia, J.C. y Aznar Martín, J. (2000). Use of real-time PCR and fluorimetry for rapid detection of rifampin and isoniazid resistance-associated mutations in Mycobacterium tuberculosis. Journal of Clinical Microbiology, 38 (9), 3194-3199.
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URI: http://hdl.handle.net/11441/64866

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