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Identification and characterization of a new porin gene of Klebsiella pneumoniae: Its role in β-lactam antibiotic resistance

Opened Access Identification and characterization of a new porin gene of Klebsiella pneumoniae: Its role in β-lactam antibiotic resistance
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Autor: Doménech Sánchez, Antonio
Hernández Allés, Santiago
Martínez Martínez, Luis
Benedí, Vicente J.
Albertí, Sebastián
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 1999
Publicado en: Journal of Bacteriology, 181 (9), 2726-2732.
Tipo de documento: Artículo
Resumen: Klebsiella pneumoniae porin genes were analyzed to detect mutations accounting for the porin deficiency observed in many β-lactam-resistant strains. PCR and Southern blot analysis revealed the existence of a third porin gene in addition to the OmpK36 and OmpK35 porin genes previously described. This new porin gene was designated ompK37 and is present in all of the clinical isolates tested. The OmpK37 porin gene was cloned, sequenced, and overexpressed in Escherichia coli. In contrast to that of the major potins, OmpK37 porin expression was only detectable by Western blot analysis in porin-deficient β-lactam-resistant strains, suggesting strong down regulation under standard laboratory conditions. Functional characterization suggested a narrower pore for the OmpK37 porin than for K. pneumoniae porins OmpK36 and OmpK35. This correlated with the susceptibility to certain β- lactam antibiotics, since a K. pneumoniae strain expressing porin OmpK37, but not porin OmpK36 or OmpK35, was less ...
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Cita: Doménech Sánchez, A., Hernández Allés, S., Martínez Martínez, L., Benedí, V.J. y Albertí, S. (1999). Identification and characterization of a new porin gene of Klebsiella pneumoniae: Its role in β-lactam antibiotic resistance. Journal of Bacteriology, 181 (9), 2726-2732.
Tamaño: 448.2Kb
Formato: PDF

URI: http://hdl.handle.net/11441/64856

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