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Uptake and intracellular activity of an optically active ofloxacin isomer in human neutrophils and tissue culture cells
dc.creator | Pascual Hernández, Álvaro | es |
dc.creator | García Luque, Isabel | es |
dc.creator | Perea Pérez, Evelio José | es |
dc.date.accessioned | 2017-09-28T16:17:24Z | |
dc.date.available | 2017-09-28T16:17:24Z | |
dc.date.issued | 1990-01-01 | |
dc.identifier.citation | Pascual Hernández, Á., García Luque, I. y Perea Pérez, E.J. (1990). Uptake and intracellular activity of an optically active ofloxacin isomer in human neutrophils and tissue culture cells. Antimicrobial Agents and Chemotherapy, 34 (2), 277-280. | |
dc.identifier.issn | 0066-4804 (impreso) | es |
dc.identifier.issn | 1098-6596 (electrónico) | es |
dc.identifier.uri | http://hdl.handle.net/11441/64846 | |
dc.description.abstract | The penetration of an optically active ofloxacin isomer [(-)-ofloxacin] into human neutrophils and different tissue culture cells (HEp-2, McCoy, MDCK, and Vero) was studied and compared with that of ofloxacin by a fluorometric assay. The cellular-to-extracellular-concentration ratios (C/E) of (-)-ofloxacin were always higher than 6, significantly greater than those of ofloxacin at extracellular concentrations of 5 and 10 mg/liter. The penetration of (-)-ofloxacin and ofloxacin was doubled when neutrophils were stimulated by phorbol myristate acetate but not affected after ingestion of opsonized Staphylococcus aureus. The C/E ratios of (-)-ofloxacin and ofloxacin for different tissue culture epithelial cells and fibroblasts were lower than those of neutrophils but still higher than 2. Both compounds produced a significant reduction in viable intraphagocytic S. aureus during 3 h of exposure to antimicrobial agents. We conclude that (-)-ofloxacin appears to reach higher intracellular concentrations than ofloxacin, remaining active inside the neutrophils. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Antimicrobial Agents and Chemotherapy, 34 (2), 277-280. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Uptake and intracellular activity of an optically active ofloxacin isomer in human neutrophils and tissue culture cells | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
idus.format.extent | 4 p. | es |
dc.journaltitle | Antimicrobial Agents and Chemotherapy | es |
dc.publication.volumen | 34 | es |
dc.publication.issue | 2 | es |
dc.publication.initialPage | 277 | es |
dc.publication.endPage | 280 | es |
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