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In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum cephalosporins

 

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dc.creator Martínez Martínez, Luis es
dc.creator Hernández Allés, Santiago es
dc.creator Albertí, Sebastián es
dc.creator Tomás, Juan M. es
dc.creator Benedí, Vicente J. es
dc.creator Jacoby, George A. es
dc.date.accessioned 2017-09-28T16:10:05Z
dc.date.available 2017-09-28T16:10:05Z
dc.date.issued 1996-02-01
dc.identifier.citation Martínez Martínez, L., Hernández Allés, S., Albertí, S., Tomás, J.M., Benedí, V.J. y Jacoby, G.A. (1996). In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum cephalosporins. Antimicrobial Agents and Chemotherapy, 40 (2), 342-348.
dc.identifier.issn 0066-4804 (impreso) es
dc.identifier.issn 1098-6596 (electrónico) es
dc.identifier.uri http://hdl.handle.net/11441/64843
dc.description.abstract Four Klebsiella pneumoniae isolates (LB1, LB2, LB3, and LB4) with increased antimicrobial resistance were obtained from the same patient. The four isolates were indistinguishable in biotype, plasmid content, lipopolysaccharide, and DNA analysis by pulse-field gel electrophoresis. Isolate LB1 made TEM-1 and SHV-1 β-lactamases. Isolates LB2, LB3, and LB4 produced SHV-5 in addition to TEM-1 and SHV-1. MICs of cefoxitin, ceftazidime, and cefotaxime against LB1 were 4, 1, and 0.06 μg/ml, respectively. MICs of ceftazidime against K. pneumoniae LB2, LB3, and LB4 were >256 μg/ml, and those of cefotaxime were 2, 4, and 64 μg/ml, respectively. MICs of cefoxitin against K. pneumoniae LB2 and LB3 were 4 μg/ml, but that against K. pneumoniae LB4 was 128 μg/ml. K. pneumoniae LB4 could transfer resistance to ceftazidime and cefotaxime, but not that to cefoxitin, to Escherichia coli. Isolate LB4 and cefoxitin-resistant laboratory mutants lacked an outer membrane protein of about 35 kDa whose molecular mass, mode of isolation, resistance to proteases, and reaction with a porin-specific antiserum suggested that it was a porin. MICs of cefoxitin and cefotaxime reverted to 4 and 2 μg/ml, respectively, when isolate LB4 was transformed with a gene coding for the K. pneumoniae porin OmpK36. We conclude that the increased resistance to cefoxitin and expanded-spectrum cephalosporins of isolate LB4 was due to loss of a porin channel for antibiotic uptake. es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher American Society for Microbiology es
dc.relation.ispartof Antimicrobial Agents and Chemotherapy, 40 (2), 342-348.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.title In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum cephalosporins es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Microbiología es
idus.format.extent 7 p. es
dc.journaltitle Antimicrobial Agents and Chemotherapy es
dc.publication.volumen 40 es
dc.publication.issue 2 es
dc.publication.initialPage 342 es
dc.publication.endPage 348 es
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