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N-acetylcysteine selectively antagonizes the activity of imipenem in Pseudomonas aeruginosa by an OprD-mediated mechanism

Opened Access N-acetylcysteine selectively antagonizes the activity of imipenem in Pseudomonas aeruginosa by an OprD-mediated mechanism

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Autor: Rodríguez Beltrán, Jerónimo
Cabot, Gabriel
Ynés Valencia, Estela
Costas, Coloma
Bou, Germán
Oliver, Antonio
Blázquez, Jesús
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 2015-01-01
Publicado en: Antimicrobial Agents and Chemotherapy, 59 (6), 3246-3251.
Tipo de documento: Artículo
Resumen: The modulating effect of N-acetylcysteine (NAC) on the activity of different antibiotics has been studied in Pseudomonas aeruginosa. Our results demonstrate that, in contrast to previous reports, only the activity of imipenem is clearly affected by NAC. MIC and checkerboard determinations indicate that the NAC-based modulation of imipenem activity is dependent mainly on OprD. SDS-PAGE of outer membrane proteins (OMPs) after NAC treatments demonstrates that NAC does not modify the expression of OprD, suggesting that NAC competitively inhibits the uptake of imipenem through OprD. Similar effects on imipenem activity were obtained with P. aeruginosa clinical isolates. Our results indicate that imipenem-susceptible P. aeruginosa strains become resistant upon simultaneous treatment with NAC and imipenem. Moreover, the generality of the observed effects of NAC on antibiotic activity was assessed with two additional bacterial species, Escherichia coli and Acinetobacter baumannii. Caution sho...
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Cita: Rodríguez Beltrán, J., Cabot, G., Ynés Valencia, E., Costas, C., Bou, G., Oliver, A. y Blázquez, J. (2015). N-acetylcysteine selectively antagonizes the activity of imipenem in Pseudomonas aeruginosa by an OprD-mediated mechanism. Antimicrobial Agents and Chemotherapy, 59 (6), 3246-3251.
Tamaño: 696.2Kb
Formato: PDF

URI: http://hdl.handle.net/11441/64837

DOI: 10.1128/AAC.00017-15

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