Artículo
LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model
Autor/es | Rojo, Javier
Rodriguez, María J. Aranda, Ana Fernández, Tahia D. Cubells-Baeza, Nuria Torres, María J. Gomez, Francisca Palomares, Francisca Perkins, James R. Diaz-Perales, Araceli |
Fecha de publicación | 2017-01-13 |
Fecha de depósito | 2017-08-21 |
Publicado en |
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Resumen | Pru p 3 is the major peach allergen in the Mediterranean area. It frequently elicits severe reactions, limiting its study in humans, raising the need for animal models to investigate the immunological mechanisms involved. ... Pru p 3 is the major peach allergen in the Mediterranean area. It frequently elicits severe reactions, limiting its study in humans, raising the need for animal models to investigate the immunological mechanisms involved. However, no anaphylaxis model exists for Pru p 3. We aimed to develop a model of peach anaphylaxis by sensitising mice with Pru p 3 in combination with lipopolysaccharide (LPS) as an adjuvant. Four groups of mice were sensitised intranasally: untreated; treated with Pru p 3; treated with LPS; treated with Pru p 3 + LPS. After sensitisation mice were intraperitoneally challenged with Pru p 3 and in vivo and in vitro parameters were evaluated. Only mice in the Pru p 3 + LPS group showed anaphylaxis symptoms, including a decrease in temperature. Determination of in vitro parameters showed a Th2 response with an increase of Pru p 3-specific IgE and IgG1. Moreover, at the cellular level, we found increased levels of IgE and IgG1 secreting Pru p 3-specific cells and a proliferative CD4+ T-cell response. These results demonstrate that Pru p 3-specific anaphylaxis can be generated after nasal sensitisation to Pru p 3 in combination with LPS. This is a promising model for evaluating food allergy immunotherapies. |
Identificador del proyecto | PI12 / 02481
PI15 / 00559 RD 07/0064 RD12 / 0013/0001 RD07 / 0064/0003 RD12 / 0013/0016 CTS-7433 C-0044-2012 SAS2013 Ref. CD14 / 00242 BIO2013- 41403-R |
Cita | Rojo, J., Rodriguez, M.J., Aranda, A., Fernandez, T.D., Cubells-Baeza, N., Torres, M.J.,...,Diaz-Perales, A. (2017). LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model. Scientific Reports, 7 (40449) |
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