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LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model

Opened Access LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model

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Autor: Rojo, Javier
Rodriguez, Maria J.
Aranda, Ana
Fernandez, Tahia D.
Cubells-Baeza, Nuria
Torres, Maria J.
Gomez, Francisca
Palomares, Francisca
Perkins, James R.
Diaz-Perales, Araceli
Fecha: 2017-01-13
Publicado en: Scientific Reports, 7 (40449)
Tipo de documento: Artículo
Resumen: Pru p 3 is the major peach allergen in the Mediterranean area. It frequently elicits severe reactions, limiting its study in humans, raising the need for animal models to investigate the immunological mechanisms involved. However, no anaphylaxis model exists for Pru p 3. We aimed to develop a model of peach anaphylaxis by sensitising mice with Pru p 3 in combination with lipopolysaccharide (LPS) as an adjuvant. Four groups of mice were sensitised intranasally: untreated; treated with Pru p 3; treated with LPS; treated with Pru p 3 + LPS. After sensitisation mice were intraperitoneally challenged with Pru p 3 and in vivo and in vitro parameters were evaluated. Only mice in the Pru p 3 + LPS group showed anaphylaxis symptoms, including a decrease in temperature. Determination of in vitro parameters showed a Th2 response with an increase of Pru p 3-specific IgE and IgG1. Moreover, at the cellular level, we found increased levels of IgE and IgG1 secreting Pru p 3-specific cells and a prol...
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Cita: Rojo, J., Rodriguez, M.J., Aranda, A., Fernandez, T.D., Cubells-Baeza, N., Torres, M.J.,...,Diaz-Perales, A. (2017). LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model. Scientific Reports, 7 (40449)
Tamaño: 584.0Kb
Formato: PDF

URI: http://hdl.handle.net/11441/63931

DOI: 10.1038/srep40449

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