dc.creator | Cantón, Rafael | es |
dc.creator | Loza, Elena | es |
dc.creator | Cornejo Gonzalo, María Carmen | es |
dc.creator | Baquero, Fernando | es |
dc.creator | Martínez Martínez, Luis | es |
dc.date.accessioned | 2017-07-27T11:48:00Z | |
dc.date.available | 2017-07-27T11:48:00Z | |
dc.date.issued | 2003 | |
dc.identifier.citation | Cantón, R., Loza, E., Cornejo Gonzalo, M.C., Baquero, F. y Martínez Martínez, L. (2003). Quality control for β-lactam susceptibility testing with a well-defined collection of Enterobacteriaceae and Pseudomonas aeruginosa strains in Spain. Journal of Clinical Microbiology, 41 (5), 1912-1918. | |
dc.identifier.issn | 0095-1137 (impreso) | es |
dc.identifier.issn | 1098-660X (electronico) | es |
dc.identifier.uri | http://hdl.handle.net/11441/63349 | |
dc.description.abstract | Eighteen Enterobacteriaceae and Pseudomonas aeruginosa strains, 16 of them with well-defined β-lactam re sistance mechanisms, were sent to 52 Spanish microbiology laboratories. Interpretative categories for 8 extended-spectrum β-lactams were collected. Participating laboratories used their own routine susceptibility testing procedures (88% automatic systems, 10% disk diffusion, and 2% agar dilution). Control results were established by two independent reference laboratories by applying the NCCLS microdilution method and interpretative criteria. Interpretative discrepancies were observed in 16% of the results (4.4% for cefepime, 3.0% for aztreonam, 2.8% for piperacillin-tazobactam, 1.7% for cefotaxime [CTX] and ceftazidime, 1.1% for ceftriaxone, 0.9% for meropenem, and 0.3% for imipenem). High consistency with reference values (<5% of major plus very major errors) was observed with (i) American Type Culture Collection quality control strains; (ii) strains with low-efficiency mechanisms inactivating extended-spectrum β-lactams, such as OXA-1-producing Escherichiacoli or SHV-1-hyperproducing Klebsiella pneumoniae; (iii) strains with highly efficient mechanisms, such as SHV-5 porin-deficient K. pneumoniae, CTX-M-10 in Enterobacter cloacae hyperproducing AmpC, and P. aeruginosa with the MexAB OprM efflux phenotype or hyperproducing AmpC. Low consistency (>30% major plus very major errors) was detected in K1-producing Klebsiella oxytoca, CTX-M-9-producing E. coli, and in OprD- P. aeruginosa strains. Extended- spectrum β-lactamase (ESBL) -producing strains accounted for 86% of very major errors. Recognition of the ESBL phenotype was particularly low in Enterobacter cloacae strains (<35%), due to the lack of NCCLS-specific rules in this genus. A K1-producing K. oxytoca was misidentified by 10% of laboratories as an ESBL producer. The use of well-defined resistant strains is useful for improving proficiency in susceptibility testing in clinical laboratories. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Journal of Clinical Microbiology, 41 (5), 1912-1918. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Quality control for β-lactam susceptibility testing with a well-defined collection of Enterobacteriaceae and Pseudomonas aeruginosa strains in Spain | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/JCM.41.5.1912-1918.2003 | es |
dc.identifier.doi | 10.1128/JCM.41.5.1912-1918.2003 | es |
idus.format.extent | 7 p. | es |
dc.journaltitle | Journal of Clinical Microbiology | es |
dc.publication.volumen | 41 | es |
dc.publication.issue | 5 | es |
dc.publication.initialPage | 1912 | es |
dc.publication.endPage | 1918 | es |