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Quality control for β-lactam susceptibility testing with a well-defined collection of Enterobacteriaceae and Pseudomonas aeruginosa strains in Spain

Opened Access Quality control for β-lactam susceptibility testing with a well-defined collection of Enterobacteriaceae and Pseudomonas aeruginosa strains in Spain

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Autor: Cantón, Rafael
Loza, Elena
Cornejo Gonzalo, María Carmen
Baquero, Fernando
Martínez Martínez, Luis
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 2003
Publicado en: Journal of Clinical Microbiology, 41 (5), 1912-1918.
Tipo de documento: Artículo
Resumen: Eighteen Enterobacteriaceae and Pseudomonas aeruginosa strains, 16 of them with well-defined β-lactam re sistance mechanisms, were sent to 52 Spanish microbiology laboratories. Interpretative categories for 8 extended-spectrum β-lactams were collected. Participating laboratories used their own routine susceptibility testing procedures (88% automatic systems, 10% disk diffusion, and 2% agar dilution). Control results were established by two independent reference laboratories by applying the NCCLS microdilution method and interpretative criteria. Interpretative discrepancies were observed in 16% of the results (4.4% for cefepime, 3.0% for aztreonam, 2.8% for piperacillin-tazobactam, 1.7% for cefotaxime [CTX] and ceftazidime, 1.1% for ceftriaxone, 0.9% for meropenem, and 0.3% for imipenem). High consistency with reference values (<5% of major plus very major errors) was observed with (i) American Type Culture Collection quality control strains; (ii) strains with low-efficiency mechanisms...
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Cita: Cantón, R., Loza, E., Cornejo Gonzalo, M.C., Baquero, F. y Martínez Martínez, L. (2003). Quality control for β-lactam susceptibility testing with a well-defined collection of Enterobacteriaceae and Pseudomonas aeruginosa strains in Spain. Journal of Clinical Microbiology, 41 (5), 1912-1918.
Tamaño: 85.27Kb
Formato: PDF

URI: http://hdl.handle.net/11441/63349

DOI: 10.1128/JCM.41.5.1912-1918.2003

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