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Nitrate assimilation gene cluster from the heterocyst-forming cyanobacterium Anabaena sp. Strain PCC 7120

 

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Opened Access Nitrate assimilation gene cluster from the heterocyst-forming cyanobacterium Anabaena sp. Strain PCC 7120
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Author: Frías Sánchez, José Enrique
Flores García, Enrique
Herrero Moreno, Antonia
Department: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Date: 1997
Published in: Journal of Bacteriology, 179 (2), 477-486.
Document type: Article
Abstract: A region of the genome of the filamentous, nitrogen-fixing, heterocyst- forming cyanobacterium Anabaena sp. strain PCC 7120 that contains a cluster of genes involved in nitrate assimilation has been identified. The genes nir, encoding nitrite reductase, and nrtABC, encoding elements of a nitrate permease, have been cloned. Insertion of a gene cassette into the nir-nrtA region impaired expression of narB, the nitrate reductase structural gene which together with nrtD is found downstream from nrtC in the gene cluster. This indicates that the nir-nrtABCD-narB genes are cotranscribed, thus constituting an operon. Expression of the nir operon in strain PCC 7120 is subjected to ammonium-promoted repression and takes place from an NtcA- activated promoter located 460 bp upstream from the start of the nir gene. In the absence of ammonium, cellular levels of the products of the nir operon are higher in the presence of nitrate than in the absence of combined nitrogen.
Cite: Frías, J.E., Flores García, E. y Herrero, A. (1997). Nitrate assimilation gene cluster from the heterocyst-forming cyanobacterium Anabaena sp. Strain PCC 7120. Journal of Bacteriology, 179 (2), 477-486.
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URI: http://hdl.handle.net/11441/63086

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