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Localized induction of the ntcA regulatory gene in developing heterocysts of Anabaena sp. strain PCC 7120

Opened Access Localized induction of the ntcA regulatory gene in developing heterocysts of Anabaena sp. strain PCC 7120

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Autor: Olmedo Verd, Elvira
Muro Pastor, Alicia María
Flores García, Enrique
Herrero Moreno, Antonia
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2006
Publicado en: Journal of Bacteriology, 188 (18), 6694-6699.
Tipo de documento: Artículo
Resumen: The ntcA gene encodes an N-control transcriptional regulator in cyanobacteria. In the N2-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, ntcA is an autoregulatory gene that is transcribed from a complex promoter region that includes a constitutive promoter (P 2) and promoters that are induced upon N step-down (P1 and P3). Expression of ntcA was investigated with the use of an ntcA-gfp translational fusion, which was introduced both in the natural ntcA locus and in a heterologous genomic place. Induction of ntcA-gfp took place after N step-down in all the cells of the filament, but at especially high levels in developing heterocysts. Localized induction could be driven independently by P3 and P1.
Cita: Olmedo Verd, E., Muro Pastor, A.M., Flores García, E. y Herrero, A. (2006). Localized induction of the ntcA regulatory gene in developing heterocysts of Anabaena sp. strain PCC 7120. Journal of Bacteriology, 188 (18), 6694-6699.
Tamaño: 402.5Kb
Formato: PDF

URI: http://hdl.handle.net/11441/62965

DOI: 10.1128/JB.00509-06

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