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Posttranscriptional regulation of glutamine synthetase in the filamentous cyanobacterium Anabaena sp. PCC 7120: Differential expression between vegetative cells and heterocysts

Opened Access Posttranscriptional regulation of glutamine synthetase in the filamentous cyanobacterium Anabaena sp. PCC 7120: Differential expression between vegetative cells and heterocysts

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Autor: Galmozzi, Carla V.
Saelices Gómez, Lorena
Florencio Bellido, Francisco Javier
Muro Pastor, María Isabel
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2010
Publicado en: Journal of Bacteriology, 192 (18), 4701-4711.
Tipo de documento: Artículo
Resumen: Genes homologous to those implicated in glutamine synthetase (GS) regulation by protein-protein interaction in the cyanobacterium Synechocystis sp. strain PCC 6803 are conserved in several cyanobacterial sequenced genomes. We investigated this GS regulatory mechanism in Anabaena sp. strain PCC 7120. In this strain the system operates with only one GS inactivation factor (inactivation factor 7A [IF7A]), encoded by open reading frame (ORF) asl2329 (gifA). Following addition of ammonium, expression of gifA is derepressed, leading to the synthesis of IF7A, and consequently, GS is inactivated. Upon ammonium removal, the GS activity returns to the initial level and IF7A becomes undetectable. The global nitrogen control protein NtcA binds to the gifA promoter. Constitutive high expression levels of gifA were found in an Anabaena ntcA mutant (CSE2), indicating a repressor role for NtcA. In vitro studies demonstrate that Anabaena GS is not inactivated by Synechocystis IFs (IF7 and IF17), indic...
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Cita: Galmozzi, C.V., Saelices Gómez, L., Florencio Bellido, F.J. y Muro Pastor, M.I. (2010). Posttranscriptional regulation of glutamine synthetase in the filamentous cyanobacterium Anabaena sp. PCC 7120: Differential expression between vegetative cells and heterocysts. Journal of Bacteriology, 192 (18), 4701-4711.
Tamaño: 1.758Mb
Formato: PDF

URI: http://hdl.handle.net/11441/62900

DOI: 10.1128/JB.00222-10

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