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Regulation of internal promoters in a zinc-responsive operon is influenced by transcription from upstream promoters

Opened Access Regulation of internal promoters in a zinc-responsive operon is influenced by transcription from upstream promoters

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Autor: Napolitano, Mauro
Rubio Gómez, Miguel Ángel
Camargo Bernal, Sergio
Luque Romero, Ignacio
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2013
Publicado en: Journal of Bacteriology, 195 (6), 1285-1293.
Tipo de documento: Artículo
Resumen: In the cyanobacterium Anabaena sp. strain PCC 7120 (also known as Nostoc sp. strain PCC 7120), a zinc-responsive operon (all4725-all4721) has been described, which contains 4 distinct promoters. The two most upstream ones bind Zur with high affinity, whereas the other two do not or do so with a very low affinity. In this paper, a detailed characterization of the four promoters is presented, showing that all four were induced by metal depletion, and they were constitutively derepressed in a zur mutant, despite the two downstream promoters not being direct targets for this regulator. Crucially, induction by metal depletion of the two downstream promoters was abrogated when transcription initiated at the upstream promoters was interrupted by a polar insertion midway in the operon. In contrast, insertion of a nitrogen-responsive promoter at a roughly similar position provoked the two downstream promoters to adopt a regulatory pattern mimicking that of the inserted promoter. Thus, regulati...
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Cita: Napolitano, M., Rubio Gómez, M.Á., Camargo Bernal, S. y Luque Romero, I. (2013). Regulation of internal promoters in a zinc-responsive operon is influenced by transcription from upstream promoters. Journal of Bacteriology, 195 (6), 1285-1293.
Tamaño: 1.797Mb
Formato: PDF

URI: http://hdl.handle.net/11441/62897

DOI: 10.1128/JB.01488-12

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