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Specific role of the cyanobacterial pipX factor in the heterocysts of Anabaena sp. strain PCC 7120

Opened Access Specific role of the cyanobacterial pipX factor in the heterocysts of Anabaena sp. strain PCC 7120

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Autor: Valladares Ruiz, Ana
Rodríguez, Virginia
Camargo Bernal, Sergio
Martínez Noël, Giselle M. A.
Herrero Moreno, Antonia
Luque Romero, Ignacio
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2011
Publicado en: Journal of Bacteriology, 193 (5), 1172-1182.
Tipo de documento: Artículo
Resumen: The PipX factor is a regulatory protein that seems to occur only in cyanobacteria. In the filamentous, heterocyst-forming Anabaena sp. strain PCC 7120, open reading frame (ORF) asr0485, identified as the pipX gene, is expressed mainly under conditions of combined-nitrogen deprivation dependent on the global N regulator NtcA and the heterocyst-specific regulator HetR. Primer extension and 5′ rapid amplification of cDNA ends (RACE) analyses detected three transcription start points corresponding to a canonical NtcA-activated promoter (to which direct binding of NtcA was observed), an NtcA- and HetR-dependent promoter, and a consensus-type promoter, the last with putative -35 and -10 determinants. Activation of pipX took place in cells differentiating into heterocysts at intermediate to late stages of the process. Accordingly, disruption of pipX led to impaired diazotrophic growth, reduced nitrogenase activity, and impaired activation of the nitrogenase structural genes. The nitrogenase ...
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Cita: Valladares Ruiz, A., Rodríguez, V., Camargo Bernal, S., Martínez Noël, G.M.A., Herrero, A. y Luque Romero, I. (2011). Specific role of the cyanobacterial pipX factor in the heterocysts of Anabaena sp. strain PCC 7120. Journal of Bacteriology, 193 (5), 1172-1182.
Tamaño: 2.236Mb
Formato: PDF

URI: http://hdl.handle.net/11441/62805

DOI: 10.1128/JB.01202-10

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