Opened Access A paradoxical mutant GATA factor

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Autor: Muro Pastor, María Isabel
Strauss, Joseph
Ramón, Ana
Scazzocchio, Claudio
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2004-04
Publicado en: Eukaryotic Cell, 3 (2), 393-405.
Tipo de documento: Artículo
Resumen: The niiA (nitrite reductase) and niaD (nitrate reductase) genes of Aspergillus nidulans are subject to both induction by nitrate and repression by ammonium or glutamine. The intergenic region between these genes functions as a bidirectional promoter. In this region, nucleosomes are positioned under nonexpression conditions. On nitrate induction under derepressing conditions, total loss of positioning occurs. This is independent of transcription and of the NirA-speciflc transcription factor but absolutely dependent on the wide-domain GATA-binding AreA factor. We show here that a 3-amino-acid deletion in the basic carboxy-terminal sequence of the DNA-binding domain results in a protein with paradoxical properties. Its weak DNA binding is consistent with its loss-of-function phenotype on most nitrogen sources. However, it results in constitutive expression and superinducibility of niiA and niaD. Nucleosome loss of positioning is also constitutive. The mutation partially suppresses null m...
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Cita: Muro Pastor, M.I., Strauss, J., Ramón, A. y Scazzocchio, C. (2004). A paradoxical mutant GATA factor. Eukaryotic Cell, 3 (2), 393-405.
Tamaño: 2.297Mb
Formato: PDF

URI: http://hdl.handle.net/11441/62487

DOI: 10.1128/EC.3.2.393–405.2004

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