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Cysteine Proteases and Cell Differentiation: Excystment of the Ciliated Protist Sterkiella histriomuscorum

Opened Access Cysteine Proteases and Cell Differentiation: Excystment of the Ciliated Protist Sterkiella histriomuscorum

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Autor: Villalobo Polo, Eduardo
Moch, Clara
Fryd Versavel, Ghislaine
Fleury Aubusson, Anne
Morin, Loïc
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 2003-12
Publicado en: Eukaryotic Cell, 2 (6), 1234-1245.
Tipo de documento: Artículo
Resumen: The process of excystment of Sterkiella histriomuscorum (Ciliophora, Oxytrichidae) leads in a few hours, through a massive influx of water and the resorption of the cyst wall, from an undifferentiated resting cyst to a highly differentiated and dividing vegetative cell. While studying the nature of the genes involved in this process, we isolated three different cysteine proteases genes, namely, a cathepsin B gene, a cathepsin L-like gene, and a calpain-like gene. Excystation was selectively inhibited at a precise differentiating stage by cysteine proteases inhibitors, suggesting that these proteins are specifically required during the excystment process. Reverse transcription-PCR experiments showed that both genes display differential expression between the cyst and the vegetative cells. A phylogenetic analysis showed for the first time that the cathepsin B tree is paraphyletic and that the diverging S. histriomuscorum cathepsin B is closely related to its Giardia homologues, which ta...
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Cita: Villalobo Polo, E., Moch, C., Fryd Versavel, G., Fleury Aubusson, A. y Morin, L. (2003). Cysteine Proteases and Cell Differentiation: Excystment of the Ciliated Protist Sterkiella histriomuscorum. Eukaryotic Cell, 2 (6), 1234-1245.
Tamaño: 1.655Mb
Formato: PDF

URI: http://hdl.handle.net/11441/62486

DOI: 10.1128/EC.2.6.1234–1245.2003

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