dc.creator | Fernández-Santos, José María | es |
dc.creator | Utrilla Alcolea, José Carmelo | es |
dc.creator | Conde Amiano, Esperanza | es |
dc.creator | Hevia Alonso, Antonio | es |
dc.creator | Loda, M. | es |
dc.creator | Martín Lacave, Inés María | es |
dc.date.accessioned | 2017-05-16T07:55:31Z | |
dc.date.available | 2017-05-16T07:55:31Z | |
dc.date.issued | 2001-04-25 | |
dc.identifier.citation | Fernández-Santos, J.M., Utrilla Alcolea, J.C., Conde Amiano, E., Hevia Alonso, A., Loda, M. y Martín Lacave, I.M. (2001). Decrease in calcitonin and parathyroid hormone mRNA levels and hormone secretion under long-term hypervitaminosis D3 in rats. Histology and Histopathology, 16 (2), 407-414. | |
dc.identifier.issn | 02133911 | es |
dc.identifier.uri | http://hdl.handle.net/11441/59854 | |
dc.description.abstract | In calcium homeostasis, vitamin D3 is a potent serum calcium-raising agent which in vivo regulates both calcitonin (CT) and parathyroid hormone (PTH) gene expression. Serum calcium is the major secretagogue for CT, a hormons product whose biosynthesis is the main biological activity of thyroid Ccells. Taking advantage of this regulatory mechanism, long-term vitamin D3-induced hypercalcemia has been extensively used as a model to produce hyperactivation, hyperplasia and even proliferative lesions of C-cells, supposedly to reduce the sustained high calcium serum concentrations. We have recently demonstrated that CT serum levels did not rise after long-term hypervitaminosis D3. Moreover, C-cells did not have a proliferative response, rather a decrease in CT-producing C-cell number was observed. In order to confirm the inhibitory effect of vitamin D3 on C-cells, Wistar rats were administered vitamin D3 chronically (25,000 IUId) with or without calcium chloride (CaC12). Under these long-term vitaminD -hypercalcemic conditions, calcium, active metaboetes of vitamin 4 , CT and PTH serum concentrations were determined by RIA; CT and PTH mRNA levels were analysed by Northern blot and in situ hybridization; and, finally, the ultrastructure of calciotrophic hormone-producing cells was analysed by electron microscapy. Our results show, that, in rats, long term administration of vitamin D3 results in a decrease in hormone biosynthetic activities of both PTH and CTproducing cells, albeit at different magnitudes. Based upon these results, we conclude that hypervitaminosis D3-based methods do not stimulate C-cell activity and can not be used to induce proliferative lesions of calcitonin-producing cells. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Universidad de Murcia. Departamento de Biología Celular e Histología | es |
dc.relation.ispartof | Histology and Histopathology, 16 (2), 407-414. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Vitamin D3 | es |
dc.subject | Calcitonin | es |
dc.subject | PTH | es |
dc.subject | C cells | es |
dc.subject | Parathyroid cells | es |
dc.subject | Hypercalcemia | es |
dc.title | Decrease in calcitonin and parathyroid hormone mRNA levels and hormone secretion under long-term hypervitaminosis D3 in rats | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica | es |
idus.format.extent | 8 | es |
dc.journaltitle | Histology and Histopathology | es |
dc.publication.volumen | 16 | es |
dc.publication.issue | 2 | es |
dc.publication.initialPage | 407 | es |
dc.publication.endPage | 414 | es |