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RNA-seq analysis of the Rhizobium tropici CIAT 899 transcriptome shows similarities in the activation patterns of symbiotic genes in the presence of apigenin and salt

 

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dc.creator Pérez Montaño, Francisco de Asís es
dc.creator Cerro Sánchez, Pablo del es
dc.creator Jiménez Guerrero, Irene es
dc.creator López Baena, Francisco Javier es
dc.creator Cubo Sánchez, María Teresa es
dc.creator Hungria, Mariangela es
dc.creator Megías Guijo, Manuel es
dc.creator Ollero Márquez, Francisco Javier es
dc.date.accessioned 2017-04-28T10:55:24Z
dc.date.available 2017-04-28T10:55:24Z
dc.date.issued 2016
dc.identifier.citation Pérez Montaño, F.d.A., Cerro, P.d., Jiménez Guerrero, I., López Baena, F.J., Cubo Sánchez, M.T., Hungria, M.,...,Ollero Márquez, F.J. (2016). RNA-seq analysis of the Rhizobium tropici CIAT 899 transcriptome shows similarities in the activation patterns of symbiotic genes in the presence of apigenin and salt. BMC Genomics, 17 (198), 1-11.
dc.identifier.issn 1471-2164 es
dc.identifier.uri http://hdl.handle.net/11441/58961
dc.description.abstract Background Rhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. Conclusions To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions. es
dc.description.sponsorship España, Ministerio de Economía y Competitividad AGL2012-1 es
dc.description.sponsorship España, Junta de Andalucía P11-CVI-7050 es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher BioMed Central es
dc.relation.ispartof BMC Genomics, 17 (198), 1-11.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject RNA-seq es
dc.subject Rhizobium tropici CIAT 899 es
dc.subject Nodulation es
dc.subject Nod factors es
dc.subject Lipochitooligosaccharides es
dc.subject Apigenin es
dc.subject Salt stress es
dc.title RNA-seq analysis of the Rhizobium tropici CIAT 899 transcriptome shows similarities in the activation patterns of symbiotic genes in the presence of apigenin and salt es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Microbiología es
dc.relation.projectID info:eu-repo/grantAgreement/MINECO/AGL2012-1 es
dc.relation.projectID P11-CVI-7050 es
dc.relation.publisherversion 10.1186/s12864-016-2543-3 es
dc.identifier.doi 10.1186/s12864-016-2543-3 es
idus.format.extent 12 p. es
dc.journaltitle BMC Genomics es
dc.publication.volumen 17 es
dc.publication.issue 198 es
dc.publication.initialPage 1 es
dc.publication.endPage 11 es
dc.contributor.funder Ministerio de Economía y Competitividad (MINECO). España
dc.contributor.funder Junta de Andalucía
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