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Hydroxyurea-Stalled Replication Forks Become Progressively Inactivated and Require Two Different RAD51-Mediated Pathways for Restart and Repair

 

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dc.creator Petermann, Eva es
dc.creator Orta Vázquez, Manuel Luis es
dc.creator Issaeva, Natalia es
dc.creator Schultz, Niklas es
dc.creator Helleday, Thomas es
dc.date.accessioned 2017-04-28T10:50:43Z
dc.date.available 2017-04-28T10:50:43Z
dc.date.issued 2010
dc.identifier.citation Petermann, E., Orta Vázquez, M.L., Issaeva, N., Schultz, . y Helleday, T. (2010). Hydroxyurea-Stalled Replication Forks Become Progressively Inactivated and Require Two Different RAD51-Mediated Pathways for Restart and Repair. Molecular Cell, 37 (4), 1-11.
dc.identifier.issn 1097-2765 es
dc.identifier.uri http://hdl.handle.net/11441/58958
dc.description.abstract Faithful DNA replication is essential to all life. Hydroxyurea (HU) depletes the cells of dNTPs, which initially results in stalled replication forks that, after prolonged treatment, collapse into DSBs. Here, we report that stalled replication forks are efficiently restarted in a RAD51-dependent process that does not trigger homologous recombination (HR). The XRCC3 protein, which is required for RAD51 foci formation, is also required for replication restart of HU-stalled forks, suggesting that RAD51-mediated strand invasion supports fork restart. In contrast, replication forks collapsed by prolonged replication blocks do not restart, and global replication is rescued by new origin firing. We find that RAD51-dependent HR is triggered for repair of collapsed replication forks, without apparent restart. In conclusion, our data suggest that restart of stalled replication forks and HR repair of collapsed replication forks require two distinct RAD51-mediated pathways. es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Elsevier es
dc.relation.ispartof Molecular Cell, 37 (4), 1-11.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject DNA es
dc.title Hydroxyurea-Stalled Replication Forks Become Progressively Inactivated and Require Two Different RAD51-Mediated Pathways for Restart and Repair es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Biología Celular es
dc.relation.publisherversion http://dx.doi.org/10.1016/j.molcel.2010.01.021 es
dc.identifier.doi 10.1016/j.molcel.2010.01.021 es
idus.format.extent 12 p. es
dc.journaltitle Molecular Cell es
dc.publication.volumen 37 es
dc.publication.issue 4 es
dc.publication.initialPage 1 es
dc.publication.endPage 11 es
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