dc.creator | Sánchez Riego, Ana María | es |
dc.creator | López Maury, Luis | es |
dc.creator | Florencio Bellido, Francisco Javier | es |
dc.date.accessioned | 2017-04-07T07:41:23Z | |
dc.date.available | 2017-04-07T07:41:23Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Sánchez Riego, A.M., López Maury, L. y Florencio Bellido, F.J. (2014). Genomic responses to arsenic in the cyanobacterium Synechocystis sp. PCC 6803. Plos One, 9 (5), e96826-1-e96826-13. | |
dc.identifier.issn | 1932-6203 | es |
dc.identifier.uri | http://hdl.handle.net/11441/57325 | |
dc.description.abstract | Arsenic is a ubiquitous contaminant and a toxic metalloid which presents two main redox states in nature: arsenite [AsIII] and arsenate [AsV]. Arsenic resistance in Synechocystis sp. strain PCC 6803 is mediated by the arsBHC operon and two additional arsenate reductases encoded by the arsI1 and arsI2 genes. Here we describe the genome-wide responses to the presence of arsenate and arsenite in wild type and mutants in the arsenic resistance system. Both forms of arsenic produced similar responses in the wild type strain, including induction of several stress related genes and repression of energy generation processes. These responses were transient in the wild type strain but maintained in time in an arsB mutant strain, which lacks the arsenite transporter. In contrast, the responses observed in a strain lacking all arsenate reductases were somewhat different and included lower induction of genes involved in metal homeostasis and Fe-S cluster biogenesis, suggesting that these two processes are targeted by arsenite in the wild type strain. Finally, analysis of the arsR mutant strain revealed that ArsR seems to only control 5 genes in the genome. Furthermore, the arsR mutant strain exhibited hypersentivity to nickel, copper and cadmium and this phenotype was suppressed by mutation in arsB but not in arsC gene suggesting that overexpression of arsB is detrimental in the presence of these metals in the media. | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad BFU2010– 15708 | es |
dc.description.sponsorship | Fondo Europeo de Desarrollo Regional E–CLPN09–001 | es |
dc.description.sponsorship | Junta de Andalucía BIO–284 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation.ispartof | Plos One, 9 (5), e96826-1-e96826-13. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Arsenate Reductases | es |
dc.subject | Copper | es |
dc.subject | Gene Expression Regulation | es |
dc.subject | Oligonucleotide Array Sequence Analysis | es |
dc.subject | Phenotype | es |
dc.subject | Synechocystis | es |
dc.title | Genomic responses to arsenic in the cyanobacterium Synechocystis sp. PCC 6803 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO/BFU2010–15708 | es |
dc.relation.projectID | E–CLPN09–001 | es |
dc.relation.projectID | BIO–284 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pone.0096826 | es |
dc.identifier.doi | 10.1371/journal.pone.0096826 | es |
idus.format.extent | 13 p. | es |
dc.journaltitle | Plos One | es |
dc.publication.volumen | 9 | es |
dc.publication.issue | 5 | es |
dc.publication.initialPage | e96826-1 | es |
dc.publication.endPage | e96826-13 | es |