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NopC is a rhizobium-specific type 3 secretion system effector secreted by sinorhizobium (ensifer) fredii HH103


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dc.creator Jiménez Guerrero, Irene es
dc.creator Pérez Montaño, Francisco de Asís es
dc.creator Medina Morillas, Carlos es
dc.creator Ollero Márquez, Francisco Javier es
dc.creator López Baena, Francisco Javier es 2017-03-16T13:07:50Z 2017-03-16T13:07:50Z 2015
dc.identifier.citation Jiménez Guerrero, I., Pérez Montaño, F., Medina, C., Ollero Márquez, F.J. y López Baena, F.J. (2015). NopC is a rhizobium-specific type 3 secretion system effector secreted by sinorhizobium (ensifer) fredii HH103. Plos One, 10 (11), e0142866-1-e0142866-17.
dc.identifier.issn 1932-6203 es
dc.description.abstract Sinorhizobium (Ensifer) fredii HH103 is a broad host-range nitrogen-fixing bacterium able to nodulate many legumes, including soybean. In several rhizobia, root nodulation is influenced by proteins secreted through the type 3 secretion system (T3SS). This specialized secretion apparatus is a common virulence mechanism of many plant and animal pathogenic bacteria that delivers proteins, called effectors, directly into the eukaryotic host cells where they interfere with signal transduction pathways and promote infection by suppressing host defenses. In rhizobia, secreted proteins, called nodulation outer proteins (Nops), are involved in hostrange determination and symbiotic efficiency. S. fredii HH103 secretes at least eight Nops through the T3SS. Interestingly, there are Rhizobium-specific Nops, such as NopC, which do not have homologues in pathogenic bacteria. In this work we studied the S. fredii HH103 nopC gene and confirmed that its expression was regulated in a flavonoid-, NodD1-and TtsI-dependent manner. Besides, in vivo bioluminescent studies indicated that the S. fredii HH103 T3SS was expressed in young soybean nodules and adenylate cyclase assays confirmed that NopC was delivered directly into soybean root cells by means of the T3SS machinery. Finally, nodulation assays showed that NopC exerted a positive effect on symbiosis with Glycine max cv. Williams 82 and Vigna unguiculata. All these results indicate that NopC can be considered a Rhizobium-specific effector secreted by S. fredii HH103 es
dc.description.sponsorship Junta de Andalucía P11-CVI-7050 es
dc.description.sponsorship Ministerio de Economía y Competitividad AGL2012-38831 es
dc.description.sponsorship Universidad de Sevilla es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Public Library of Science es
dc.relation.ispartof Plos One, 10 (11), e0142866-1-e0142866-17.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri *
dc.subject Adenylate cyclase assay es
dc.subject Cowpea es
dc.subject Gene expression regulation es
dc.subject Rhizobium es
dc.title NopC is a rhizobium-specific type 3 secretion system effector secreted by sinorhizobium (ensifer) fredii HH103 es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Microbiología es
dc.relation.projectID P11-CVI-7050 es
dc.relation.projectID info:eu-repo/grantAgreement/MINECO/AGL2012-38831 es
dc.relation.publisherversion es
dc.identifier.doi 10.1371/journal.pone.0142866 es
idus.format.extent 17 p. es
dc.journaltitle Plos One es
dc.publication.volumen 10 es
dc.publication.issue 11 es
dc.publication.initialPage e0142866-1 es
dc.publication.endPage e0142866-17 es
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