Artículo
Identification of a gene encoding a thioredoxin-like product necessary for cytochrome c biosynthesis and symbiotic nitrogen fixation in Rhizobium leguminosarum
Autor/es | Vargas Macías, Carmen
Wu, Guanghui Davies, Andrea E. Downie, John Allan |
Departamento | Universidad de Sevilla. Departamento de Microbiología y Parasitología |
Fecha de publicación | 1994 |
Fecha de depósito | 2016-06-23 |
Publicado en |
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Resumen | A Tn5-induced mutant of Rhizobium leguminosarum bv. viciae could not form nitrogen-fixing nodules on pea
or vetch because of a lesion in electron transport to oxygen. The mutant lacked spectroscopically detectable
cytochromes ... A Tn5-induced mutant of Rhizobium leguminosarum bv. viciae could not form nitrogen-fixing nodules on pea or vetch because of a lesion in electron transport to oxygen. The mutant lacked spectroscopically detectable cytochromes c and aa3. No proteins containing c-type cytochromes could be identified in the mutant by heme staining of proteins fractionated on polyacrylamide gels, indicating that the mutant was defective in maturation of all c-type cytochromes. The Tn5 mutation was determined to be located in a gene that was called cycY. The cycY gene product is homologous to the thioredoxin-like protein HelX involved in the assembly of c-type cytochromes in Rhodobacter capsulatus and to an open reading frame from a Bradyrhizobium japonicum gene cluster containing other genes involved in cytochrome c biogenesis. Our observations are consistent with CycY functioning as a thioredoxin that reduces cysteine residues in apocytochromes c before heme attachment |
Cita | Vargas, C., Wu, G., Davies, A.E. y Downie, J.A. (1994). Identification of a gene encoding a thioredoxin-like product necessary for cytochrome c biosynthesis and symbiotic nitrogen fixation in Rhizobium leguminosarum. Journal of Bacteriology, 176 (13), 4117-4123. |
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