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Identification of a gene encoding a thioredoxin-like product necessary for cytochrome c biosynthesis and symbiotic nitrogen fixation in Rhizobium leguminosarum

Opened Access Identification of a gene encoding a thioredoxin-like product necessary for cytochrome c biosynthesis and symbiotic nitrogen fixation in Rhizobium leguminosarum
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Autor: Vargas, C.
Wu, Guanghui
Davies, Andrea E.
Downie, John Allan
Departamento: Universidad de Sevilla. Departamento de Microbiología y Parasitología
Fecha: 1994
Publicado en: Journal of Bacteriology, 176 (13), 4117-4123.
Tipo de documento: Artículo
Resumen: A Tn5-induced mutant of Rhizobium leguminosarum bv. viciae could not form nitrogen-fixing nodules on pea or vetch because of a lesion in electron transport to oxygen. The mutant lacked spectroscopically detectable cytochromes c and aa3. No proteins containing c-type cytochromes could be identified in the mutant by heme staining of proteins fractionated on polyacrylamide gels, indicating that the mutant was defective in maturation of all c-type cytochromes. The Tn5 mutation was determined to be located in a gene that was called cycY. The cycY gene product is homologous to the thioredoxin-like protein HelX involved in the assembly of c-type cytochromes in Rhodobacter capsulatus and to an open reading frame from a Bradyrhizobium japonicum gene cluster containing other genes involved in cytochrome c biogenesis. Our observations are consistent with CycY functioning as a thioredoxin that reduces cysteine residues in apocytochromes c before heme attachment
Cita: Vargas, C., Wu, G., Davies, A.E. y Downie, J.A. (1994). Identification of a gene encoding a thioredoxin-like product necessary for cytochrome c biosynthesis and symbiotic nitrogen fixation in Rhizobium leguminosarum. Journal of Bacteriology, 176 (13), 4117-4123.
Tamaño: 1.477Mb
Formato: PDF

URI: http://hdl.handle.net/11441/42692

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