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Role of central metabolism in the osmoadaptation of the halophilic bacterium chromohalobacter salexigens

Opened Access Role of central metabolism in the osmoadaptation of the halophilic bacterium chromohalobacter salexigens

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Autor: Pastor, José M.
Bernal, Vicente
Salvador de Lara, Manuel
Argandoña Bertrán, Montserrat
Vargas, C.
Csonka, Laszlo N.
Sevilla, Ángel
Iborra, José Luis
Nieto Gutiérrez, Joaquín José
Cánovas, Manuel
Departamento: Universidad de Sevilla. Departamento de Microbiología y Parasitología
Fecha: 2013
Publicado en: Journal of Biological Chemistry, 288 (24), 17769-17781.
Tipo de documento: Artículo
Resumen: Bacterial osmoadaptation involves the cytoplasmic accumulation of compatible solutes to counteract extracellular osmolarity. The halophilic and highly halotolerant bacterium Chromohalobacter salexigens is able to grow up to 3 M NaCl in a minimal medium due to the de novo synthesis of ectoines. This is an osmoregulated pathway that burdens central metabolic routes by quantitatively drawing off TCA cycle intermediaries. Consequently, metabolism in C. salexigens has adapted to support this biosynthetic route. Metabolism of C. salexigens is more efficient at high salinity than at low salinity, as reflected by lower glucose consumption, lower metabolite overflow, and higher biomass yield. At low salinity, by-products (mainly gluconate, pyruvate, and acetate) accumulate extracellularly. Using [1-13C]-, [2-13C]-, [6- 13C]-, and [U-13C6]glucose as carbon sources, we were able to determine the main central metabolic pathways involved in ectoines biosynthesis from glucose. C. salexigens uses th...
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Cita: Pastor, J.M., Bernal, V., Salvador de Lara, M., Argandoña Bertrán, M., Vargas, C., Csonka, L.N.,...,Cánovas, M. (2013). Role of central metabolism in the osmoadaptation of the halophilic bacterium chromohalobacter salexigens. Journal of Biological Chemistry, 288 (24), 17769-17781.
Tamaño: 1.561Mb
Formato: PDF

URI: http://hdl.handle.net/11441/42459

DOI: 10.1074/jbc.M113.470567

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