dc.creator | Martín Rubio, María Esther | es |
dc.creator | Hidalgo Jiménez, Josefina | es |
dc.creator | Rosa, José Luis | es |
dc.creator | Crottet, Pascal | es |
dc.creator | Velasco López, Ángel | es |
dc.date.accessioned | 2016-05-03T11:52:21Z | |
dc.date.available | 2016-05-03T11:52:21Z | |
dc.date.issued | 2000 | |
dc.identifier.citation | Martín Rubio, M.E., Hidalgo Jiménez, J., Rosa, J.L., Crottet, P. y Velasco López, Á. (2000). Effect of protein kinase a activity on the association of ADP-ribosylation factor 1 to Golgi membranes. The Journal of the Biological Chemistry, 275 (25), 19050-19059. | |
dc.identifier.issn | 1083-351X | es |
dc.identifier.uri | http://hdl.handle.net/11441/40671 | |
dc.description.abstract | The small GTP-binding protein ADP-ribosylation factor
1 (ARF1) is an essential component of the molecular
machinery that catalyzes the formation of membranebound
transport intermediates. By using an in vitro assay
that reproduces recruitment of cytosolic proteins
onto purified, high salt-washed Golgi membranes, we
have analyzed the role of cAMP-dependent protein kinase
A (PKA) on ARF1 incorporation. Addition to this
assay of either pure catalytic subunits of PKA (C-PKA)
or cAMP increased ARF1 binding. By contrast, ARF1
association was inhibited following C-PKA inactivation
with either PKA inhibitory peptide or RIIa as well as
after cytosol depletion of C-PKA. C-PKA also stimulated
recruitment and activation of a recombinant form of
human ARF1 in the absence of additional cytosolic components.
The binding step could be dissociated from the
activation reaction and found to be independent of guanine
nucleotides and saturable. This step was stimulated
by C-PKA in an ATP-dependent manner. Dephosphorylated
Golgi membranes exhibited a decreased
ability to recruit ARF1, and this effect was reverted by
addition of C-PKA. Following an increase in the intracellular
level of cAMP, ARF proteins redistributed from
cytosol to the perinuclear Golgi region of intact cells.
Collectively, the results show that PKA exerts a key
regulatory role in the recruitment of ARF1 onto Golgi
membranes. In contrast, PKA modulators did not affect
recruitment of b-COP onto Golgi membranes containing
prebound ARF1. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | The American Society for Biochemistry and Molecular Biology | es |
dc.relation.ispartof | The Journal of the Biological Chemistry, 275 (25), 19050-19059. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Effect of protein kinase a activity on the association of ADP-ribosylation factor 1 to Golgi membranes | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Celular | es |
dc.relation.publisherversion | http://www.jbc.org/content/275/25/19050.full.pdf+html | es |
dc.relation.publisherversion | http://dx.doi.org/10.1074/jbc.275.25.19050 | |
dc.identifier.doi | 10.1074/jbc.275.25.19050 | |
idus.format.extent | 11 | es |
dc.journaltitle | The Journal of the Biological Chemistry | |
dc.publication.volumen | 275 | |
dc.publication.issue | 25 | |
dc.publication.initialPage | 19050 | |
dc.publication.endPage | 19059 | |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/40671 | |