dc.contributor.advisor | | |
dc.creator | Muñiz Guinea, Manuel | es |
dc.creator | Martín Rubio, María Esther | es |
dc.creator | Hidalgo Jiménez, Josefina | es |
dc.creator | Velasco López, Ángel | es |
dc.date.accessioned | 2016-04-27T11:41:53Z | |
dc.date.available | 2016-04-27T11:41:53Z | |
dc.date.issued | 1997 | |
dc.identifier.citation | Muñiz Guinea, M., Martín Rubio, M.E., Hidalgo Jiménez, J. y Velasco López, Á. (1997). Protein kinase A activity is required for the budding of constitutive transport vesicles from the trans-Golgi network. Procedings of the National Academy of Sciences of the United States of America, 94 (26), 14461-14466. | |
dc.identifier.issn | 1091-6490 | es |
dc.identifier.uri | http://hdl.handle.net/11441/40526 | |
dc.description.abstract | We have examined the role played by protein kinase A (PKA) in vesicle-mediated protein transport from the trans-Golgi network (TGN) to the cell surface. In vivo this transport step was inhibited by inhibitors of PKA catalytic subunits (C-PKA) such as the compound known as H89 and a myristoylated form of the inhibitory peptide sequence contained in the thermostable PKA inhibitor. Inhibition by H89 occurred at an early stage during the transfer of vesicular stomatitis virus G glycoprotein from the TGN to the cell surface. Reversal from this inhibition correlated with a transient increase in the number of free coated vesicles in the Golgi area. Vesicle budding from the TGN was studied in vitro using vesicular stomatitis virus-infected, permeabilized cells. Addition to this assay of C-PKA stimulated vesicle release while it was suppressed by PKA inhibitory peptide, H89, and antibody against C-PKA. Furthermore, vesicle release was decreased when PKA-depleted cytosol was used and restored by addition of C-PKA. These results indicate a regulatory role for PKA activity in the production of constitutive transport vesicles from the TGN. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | National Academy of Sciences of the USA | es |
dc.relation.ispartof | Procedings of the National Academy of Sciences of the United States of America, 94 (26), 14461-14466. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Protein kinase A activity is required for the budding of constitutive transport vesicles from the trans-Golgi network | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Celular | es |
dc.relation.publisherversion | https://www.pnas.org/content/94/26/14461 | es |
dc.identifier.doi | 10.1073/pnas.94.26.14461 | |
idus.format.extent | 6 | es |
dc.journaltitle | Procedings of the National Academy of Sciences of the United States of America | |
dc.publication.volumen | 94 | |
dc.publication.issue | 26 | |
dc.publication.initialPage | 14461 | |
dc.publication.endPage | 14466 | |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/40526 | |