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Golgi structural stability and biogenesis depend on associated PKA activity

Opened Access Golgi structural stability and biogenesis depend on associated PKA activity

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Autor: Bejarano Fernández, Eloy
Cabrera Sánchez, Margarita
Vega, Lucía
Hidalgo Jiménez, Josefina
Velasco López, Ángel
Departamento: Universidad de Sevilla. Departamento de Biología Celular
Fecha: 2006
Publicado en: Journal of Cell Science, 119, 3764-3775
Tipo de documento: Artículo
Resumen: The mammalian Golgi complex consists of stacks of cisternae linked laterally into a continuous perinuclear ribbon structure. Protein kinase A is stably associated with the Golgi complex during interphase. To analyze its role in Golgi structural maintenance cells were depleted of protein kinase A regulatory subunits using small interfering RNAs. Under these conditions, the catalytic subunits redistributed to the cytosol and the entire Golgi complex underwent disassembly into multiple juxtanuclear fragments. A similar effect took place following pharmacological inhibition or redistribution of the complete holoenzyme to the cytosol. Golgi fragments maintained their polarization and competence for anterograde protein trafficking. By electron microscopy, they were identified as whorl-like structures composed of concentrically arrayed cisternae. To test a possible role of protein kinase A in Golgi biogenesis we analyzed its involvement during Golgi reassembly from the endoplasmic reticulum....
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Tamaño: 3.243Mb
Formato: PDF

URI: http://hdl.handle.net/11441/40466

DOI: http://dx.doi.org/10.1242/jcs.03146

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