dc.creator | Buendía Clavería, Ana María | es |
dc.creator | Moussaid, Ahmed | es |
dc.creator | Ollero Márquez, Francisco Javier | es |
dc.creator | Vinardell González, José María | es |
dc.creator | Torres Rueda, Antonio Ildefonso | es |
dc.creator | Moreno Onorato, Francisco Javier | es |
dc.creator | Gil Serrano, Antonio Miguel | es |
dc.creator | Rodríguez Carvajal, Miguel Ángel | es |
dc.creator | Tejero Mateo, María Pilar | es |
dc.creator | Ruiz Sainz, José Enrique | es |
dc.date.accessioned | 2016-04-22T07:05:43Z | |
dc.date.available | 2016-04-22T07:05:43Z | |
dc.date.issued | 2003 | |
dc.identifier.citation | Buendía Clavería, A.M., Moussaid, A., Ollero Márquez, F.J., Vinardell González, J.M., Torres Rueda, A.I., Moreno Onorato, F.J.,...,Ruiz Sainz, J.E. (2003). A purL mutant of Sinorhizobium fredii HH103 is symbiotically defective and altered in its lipopolysaccharide. Microbiology, 149 (7), 1807-1818. | |
dc.identifier.issn | 1465-2080 | es |
dc.identifier.issn | 1350-0872 | es |
dc.identifier.uri | http://hdl.handle.net/11441/40272 | |
dc.description.abstract | The pleiotropic phenotype of an auxotrophic purL mutant (SVQ295) of Sinorhizobium fredii HH103
has been investigated. SVQ295 forms colonies that are translucent, produce more slime and
absorb less Congo red than those of wild-type strain HH103. SVQ295 did not grow in minimal
medium unless the culture was supplemented with thiamin and adenine or with thiamin and
AICA-riboside (5-aminoimidazole-4-carboxamide 1-b-D-ribofuranoside), an intermediate of purine
biosynthesis. Bacterial cultures supplemented with AICA-riboside or adenine reached the same
culture density, although the doubling time of SVQ295 cultures containing AICA-riboside was
clearly longer. S. fredii SVQ295 induced pseudonodules on Glycine max and failed to nodulate
six different legumes. On Glycyrrhiza uralensis, however, nodules showing nitrogenase activity and
containing infected plant cells were formed. SVQ295 showed auto-agglutination when grown
in liquid TY medium and its lipopolysaccharide (LPS) electrophoretic profile differed from that of its
parental strain HH103-1. In addition, four monoclonal antibodies that recognize the LPS of S. fredii
HH103 failed to recognize the LPS produced by SVQ295. In contrast, 1H-NMR spectra of
K-antigen capsular polysaccharides (KPS) produced by SVQ295 and the wild-type strain HH103
were similar. Co-inoculation of soybean plants with SVQ295 and SVQ116 (a nodA mutant
derivative of HH103) produced nitrogen-fixing nodules that were only occupied by SVQ116. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Microbiology Society | es |
dc.relation.ispartof | Microbiology, 149 (7), 1807-1818. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | A purL mutant of Sinorhizobium fredii HH103 is symbiotically defective and altered in its lipopolysaccharide | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Celular | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química orgánica | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.publisherversion | http://www.microbiologyresearch.org/docserver/fulltext/micro/149/7/1807.pdf?expires=1461241526&id=id&accname=guest&checksum=6CC6F779AFCA324495E83AFAEEB7695A | es |
dc.identifier.doi | 10.1099/mic.0.26099-0 | es |
idus.format.extent | 12 | es |
dc.journaltitle | Microbiology | |
dc.publication.volumen | 149 | |
dc.publication.issue | 7 | |
dc.publication.initialPage | 1807 | |
dc.publication.endPage | 1818 | |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/40272 | |