Repositorio de producción científica de la Universidad de Sevilla

A purL mutant of Sinorhizobium fredii HH103 is symbiotically defective and altered in its lipopolysaccharide

Opened Access A purL mutant of Sinorhizobium fredii HH103 is symbiotically defective and altered in its lipopolysaccharide

Citas

buscar en

Estadísticas
Icon
Exportar a
Autor: Buendía Clavería, Ana María
Moussaid, Ahmed
Ollero Márquez, Francisco Javier
Moreno Onorato, Francisco Javier
Gil Serrano, Antonio Miguel
Rodríguez Carvajal, Miguel Ángel
Tejero Mateo, María Pilar
Peart, Jan L.
Brewin, Nicholas J.
Ruiz Sainz, José Enrique
Departamento: Universidad de Sevilla. Departamento de Biología Celular
Universidad de Sevilla. Departamento de Química orgánica
Universidad de Sevilla. Departamento de Microbiología
Fecha: 2003
Publicado en: Microbiology,149,1807–1818
Tipo de documento: Artículo
Resumen: The pleiotropic phenotype of an auxotrophic purL mutant (SVQ295) of Sinorhizobium fredii HH103 has been investigated. SVQ295 forms colonies that are translucent, produce more slime and absorb less Congo red than those of wild-type strain HH103. SVQ295 did not grow in minimal medium unless the culture was supplemented with thiamin and adenine or with thiamin and AICA-riboside (5-aminoimidazole-4-carboxamide 1-b-D-ribofuranoside), an intermediate of purine biosynthesis. Bacterial cultures supplemented with AICA-riboside or adenine reached the same culture density, although the doubling time of SVQ295 cultures containing AICA-riboside was clearly longer. S. fredii SVQ295 induced pseudonodules on Glycine max and failed to nodulate six different legumes. On Glycyrrhiza uralensis, however, nodules showing nitrogenase activity and containing infected plant cells were formed. SVQ295 showed auto-agglutination when grown in liquid TY medium and its lipopolysaccharide (LPS) electrophor...
[Ver más]
Tamaño: 468.7Kb
Formato: PDF

URI: http://hdl.handle.net/11441/40272

DOI: http://dx.doi.org/10.1099/mic.0.26099-0

Ver versión del editor

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones