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Neural progenitor cells isolated from the subventricular zone present hemichannel activity and form functional gap junctions with glial cells


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dc.creator Talaverón Aguilocho, Rocío es
dc.creator Fernández, Paola es
dc.creator Escamilla, Rosalba es
dc.creator Pastor Loro, Ángel Manuel es
dc.creator Rodríguez Matarredona, Esperanza es
dc.creator Sáez, Juan Carlos es 2016-04-18T09:17:17Z 2016-04-18T09:17:17Z 2015
dc.identifier.issn 1662-5102 es
dc.description.abstract The postnatal subventricular zone (SVZ) lining the walls of the lateral ventricles contains neural progenitor cells (NPCs) that generate new olfactory bulb interneurons. Communication via gap junctions between cells in the SVZ is involved in NPC proliferation and in neuroblast migration towards the olfactory bulb. SVZ NPCs can be expanded in vitro in the form of neurospheres that can be used for transplantation purposes after brain injury. We have previously reported that neurosphere-derived NPCs form heterocellular gap junctions with host glial cells when they are implanted after mechanical injury. To analyze functionality of NPC-glial cell gap junctions we performed dye coupling experiments in co-cultures of SVZ NPCs with astrocytes or microglia. Neurosphere-derived cells expressed mRNA for at least the hemichannel/gap junction channel proteins connexin 26 (Cx26), Cx43, Cx45 and pannexin 1 (Panx1). Dye coupling experiments revealed that gap junctional communication occurred among neurosphere cells (incidence of coupling: 100%). Moreover, hemichannel activity was also detected in neurosphere cells as evaluated in time-lapse measurements of ethidium bromide uptake. Heterocellular coupling between NPCs and glial cells was evidenced in co-cultures of neurospheres with astrocytes (incidence of coupling: 91.0 ± 4.7%) or with microglia (incidence of coupling: 71.9 ± 6.7%). Dye coupling in neurospheres and in co-cultures was inhibited by octanol, a gap junction blocker. Altogether, these results suggest the existence of functional hemichannels and gap junction channels in postnatal SVZ neurospheres. In addition, they demonstrate that SVZ-derived NPCs can establish functional gap junctions with astrocytes or microglia. Therefore, cell-cell communication via gap junctions and hemichannels with host glial cells might subserve a role in the functional integration of NPCs after implantation in the damaged brain es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Frontiers Research Foundation es
dc.relation.ispartof Frontiers in Cellular Neuroscience, 9 (411) es
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri *
dc.title Neural progenitor cells isolated from the subventricular zone present hemichannel activity and form functional gap junctions with glial cells es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Fisiología es
dc.relation.publisherversion 10.3389/fncel.2015.00411 es
dc.identifier.doi es
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