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The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigens

Opened Access The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigens

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Autor: Nieto Gutiérrez, Joaquín José
García Estepa, Raúl
Argandoña Bertrán, Montserrat
Reina Bueno, Mercedes
Capote Maínez, María Nieves
Iglesias Guerra, Fernando
Vargas, C.
Departamento: Universidad de Sevilla. Departamento de Microbiología y Parasitología
Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica
Fecha: 2006
Publicado en: Journal of Bacteriology, 188, 3774-3784
Tipo de documento: Artículo
Resumen: The halophilic bacterium Chromohalobacter salexigens synthesizes and accumulates compatible solutes in response to salt and temperature stress. 13C-nuclear magnetic resonance analysis of cells grown in minimal medium at the limiting temperature of 45°C revealed the presence of hydroxyectoine, ectoine, glutamate, trehalose (not present in cells grown at 37°C), and the ectoine precursor, N')'-acetyldiaminobutyric acid. High-performance liquid chromatography analyses showed that the levels of ectoine and hydroxyectoine were maximal during the stationary phase of growth. Accumulation of hydroxyectoine was up-regulated by salinity and temperature, whereas accumulation of ectoine was up-regulated by salinity and down-regulated by tem- perature. The ectD gene, which is involved in the conversion of ectoine to hydroxyectoine, was isolated as part of a DNA region that also contains a gene whose product belongs to the AraC-XylS family of transcriptional activators. Orthologs of ectD were found ...
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Tamaño: 1.192Mb
Formato: PDF

URI: http://hdl.handle.net/11441/38259

DOI: http://dx.doi.org/10.1128/JB.00136-06

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