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Artículo
Folate transport by prawn hepatopancreas brush-border membrane vesicles.
dc.creator | Blaya, J. A. | |
dc.creator | Muriana, Francisco J. G. | |
dc.creator | Ruiz Gutiérrez, Valentina | |
dc.creator | Vázquez Cueto, Carmen María | |
dc.creator | Bolufer González, José | |
dc.date.accessioned | 2015-09-23T12:10:11Z | |
dc.date.available | 2015-09-23T12:10:11Z | |
dc.date.issued | 1998 | |
dc.identifier.issn | 0144-8463 | es |
dc.identifier.uri | http://hdl.handle.net/11441/28777 | |
dc.description.abstract | The transport system of folic acid (Pte-Glu) b y brush-borde r membrane vesicles (BBMV ) isolated from prawn (Penaeus japonicm) hepatopancreas , was studied by measuring the uptake of Pte-Glu . This uptake was found to have two components , intravesicular transport and membrane binding . Membrane binding was not affected by the presence of a trans - membrane pH-gradient at a short incubation period . However , a transmembrane pH - gradient increased membrane binding at 6 0 min. The transpor to f Pte-Glu appeared to be carrier-mediated , was stimulated by an inwardly proton gradient (p H 5. 5 outside , 7. 4 inside ) and was unaffected by a sodium-gradient . The relationship between pH gradient-driven Pte-Glu uptake and medium Pte-Glu concentration followed saturating Michaelis-Menten kinetics . Eadie-Hofste e representation of the pH gradient-driven Pte-Glu uptake indicated a single transport system with a Km of 0.3 7 ^ Man d Vmax of 1.06pmol/mg protein/15s . These findings indicate that BBM V isolated from prawn hepatopancreas possesses a Pte - Glu transport system similar to that described in mammalian intestine. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Portland Press | es |
dc.relation.ispartof | Bioscience Reports, 18(1), 9-17 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Folate transport | es |
dc.subject | prawn | es |
dc.subject | hepatopancreas | es |
dc.subject | brush-border membrane vesicles | es |
dc.subject | kinetics | es |
dc.title | Folate transport by prawn hepatopancreas brush-border membrane vesicles. | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología | es |
dc.relation.publisherversion | http://dx.doi.org/10.1023/A:1022236616277 | es |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1022236616277 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/28777 |
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9.full.pdf | 468.4Kb | [PDF] | Ver/ | |