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Mostrando ítems 1-9 de 9
Artículo
The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants
(American Society for Microbiology, 2009)
Cotranscriptional R-loops are formed in yeast mutants of the THO complex, which functions at the interface between transcription and mRNA export. Despite the relevance of R-loops in transcription-associated recombination, the ...
Artículo
Yeast Sen1 helicase protects the genome from transcription-associated instability
(Elsevier, 2011-01-06)
Sen1 of S. cerevisiae is a known component of the NRD complex implicated in transcription termination of nonpolyadenylated as well as some polyadenylated RNA polymerase II transcripts. We now show that Sen1 helicase possesses ...
Artículo
An hpr1 point mutation that impairs transcription and mRNP biogenesis without increasing recombination
(American Society for Microbiology, 2006)
THO/TREX, a conserved eukaryotic protein complex, is a key player at the interface between transcription and mRNP metabolism. The lack of a functional THO complex impairs transcription, leads to transcriptiondependent ...
Artículo
A new connection of mRNP biogenesis and export with transcription-coupled repair
(Oxford University Press, 2007)
Although DNA repair is faster in the transcribed strand of active genes, little is known about the possible contribution of mRNP biogenesis and export in transcription-coupled repair (TCR). Interestingly, mutants of THO, ...
Artículo
Sem1 is a functional component of the nuclear pore complex–associated messenger RNA export machinery
(Rockefeller University Press, 2009)
The evolutionarily conserved protein Sem1/Dss1 is a subunit of the regulatory particle (RP) of the proteasome, and, in mammalian cells, binds the tumor suppressor protein BRCA2. Here, we describe a new function for yeast ...
Artículo
Excess of Yra1 RNA-Binding Factor Causes Transcription-Dependent Genome Instability, Replication Impairment and Telomere Shortening
(Public Library of Science, 2016)
Yra1 is an essential nuclear factor of the evolutionarily conserved family of hnRNP-like export factors that when overexpressed impairs mRNA export and cell growth. To investigate further the relevance of proper Yra1 ...
Artículo
A role for the Saccharomyces cerevisiae Rtt109 histone acetyltransferase in R-loop homeostasis and associated genome instability
(Genetics Society of America, 2022)
The stability of the genome is occasionally challenged by the formation of DNA-RNA hybrids and R-loops, which can be influenced by the chromatin context. This is mainly due to the fact that DNA-RNA hybrids hamper the ...
Artículo
Rat1 promotes premature transcription termination at R-loops
(Oxford University Press, 2024-04-24)
Certain DNA sequences can adopt a non-B form in the genome that interfere with DNA-templated processes, including transcription. Among the sequences that are intrinsically difficult to transcribe are those that tend to ...
Artículo
RNA biogenesis and RNA metabolism factors as R-loop suppressors: a hidden role in genome integrity.
(Cold Spring Harbor Laboratory Press, 2024-07)
Genome integrity relies on the accuracy of DNA metabolism, but as appreciated for more than four decades, transcription enhances mutation and recombination frequencies. More recent research provided evidence for a previously ...