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A STD-NMR Study of the Interaction of the Anabaena Ferredoxin-NADP+ Reductase with the Coenzyme
dc.creator | Antonini, Lara V. | es |
dc.creator | Peregrina, José R. | es |
dc.creator | Angulo Álvarez, Jesús | es |
dc.creator | Medina, Milagros | es |
dc.creator | Nieto, Pedro M. | es |
dc.date.accessioned | 2020-06-12T07:18:10Z | |
dc.date.available | 2020-06-12T07:18:10Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Antonini, L.V., Peregrina, J.R., Angulo Álvarez, J., Medina, M. y Nieto, P.M. (2014). A STD-NMR Study of the Interaction of the Anabaena Ferredoxin-NADP+ Reductase with the Coenzyme. Molecules, 19 (1), 672-685. | |
dc.identifier.issn | 1420-3049 | es |
dc.identifier.uri | https://hdl.handle.net/11441/97715 | |
dc.description.abstract | Ferredoxin-NADP+ reductase (FNR) catalyzes the electron transfer from ferredoxin to NADP+ via its flavin FAD cofactor. To get further insights in the architecture of the transient complexes produced during the hydride transfer event between the enzyme and the NADP+ coenzyme we have applied NMR spectroscopy using Saturation Transfer Difference (STD) techniques to analyze the interaction between FNRox and the oxidized state of its NADP+ coenzyme. We have found that STD NMR, together with the use of selected mutations on FNR and of the non-FNR reacting coenzyme analogue NAD+, are appropriate tools to provide further information about the the interaction epitope. | es |
dc.format | application/pdf | es |
dc.format.extent | 14 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Molecules, 19 (1), 672-685. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Saturation transfer difference NMR spectroscopy | es |
dc.subject | Flavoenzymes | es |
dc.subject | Hydride transfer | es |
dc.subject | Isoalloxazine-nicotinamide interactions | es |
dc.subject | CORCEMA-ST | es |
dc.title | A STD-NMR Study of the Interaction of the Anabaena Ferredoxin-NADP+ Reductase with the Coenzyme | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química orgánica | es |
dc.relation.publisherversion | https://doi.org/10.3390/molecules19010672 | es |
dc.identifier.doi | /10.3390/molecules19010672 | es |
dc.journaltitle | Molecules | es |
dc.publication.volumen | 19 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 672 | es |
dc.publication.endPage | 685 | es |
Ficheros | Tamaño | Formato | Ver | Descripción |
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A STD-NMR.pdf | 686.2Kb | [PDF] | Ver/ | |