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dc.creatorMachuca, Jesúses
dc.creatorRecacha, Estheres
dc.creatorBriales Martín, Alejandraes
dc.creatorDíaz de Alba, Paulaes
dc.creatorBlázquez, Jesúses
dc.creatorPascual, Álvaroes
dc.creatorRodríguez Martínez, José Manueles
dc.date.accessioned2020-05-06T06:25:00Z
dc.date.available2020-05-06T06:25:00Z
dc.date.issued2017
dc.identifier.citationMachuca, J., Recacha, E., Briales Martín, A., Díaz de Alba, P., Blázquez, J., Pascual, Á. y Rodríguez Martínez, J.M. (2017). Cellular Response to Ciprofloxacin in Low-Level Quinolone-Resistant Escherichia coli. Frontiers in Microbiology, 8, 1370.
dc.identifier.issn1664-302Xes
dc.identifier.urihttps://hdl.handle.net/11441/96177
dc.description.abstractBactericidal activity of quinolones has been related to a combination of DNA fragmentation, reactive oxygen species (ROS) production and programmed cell death (PCD) systems. The underlying molecular systems responsible for reducing bactericidal effect during antimicrobial therapy in low-level quinolone resistance (LLQR) phenotypes need to be clarified. To do this and also define possible new antimicrobial targets, the transcriptome profile of isogenic Escherichia coli harboring quinolone resistance mechanisms in the presence of a clinical relevant concentration of ciprofloxacin was evaluated. A marked differential response to ciprofloxacin of either up- or downregulation was observed in LLQR strains. Multiple genes implicated in ROS modulation (related to the TCA cycle, aerobic respiration and detoxification systems) were upregulated (sdhC up to 63.5-fold) in mutants with LLQR. SOS system components were downregulated (recA up to 30.7-fold). yihE, a protective kinase coding for PCD, was also upregulated (up to 5.2-fold). SdhC inhibition sensitized LLQR phenotypes (up to 1Log = 2.3 after 24 h). At clinically relevant concentrations of ciprofloxacin, gene expression patterns in critical systems to bacterial survival and mutant development were significantly modified in LLQR phenotypes. Chemical inhibition of SdhC (succinate dehydrogenase) validated modulation of ROS as an interesting target for bacterial sensitization.es
dc.description.sponsorshipMinisterio de Sanidad y Consumo, Instituto de Salud Carlos III ( PI11-00934, PI14/00940)es
dc.description.sponsorshipConsejería de Innovación, Ciencia y Empresa, Junta de Andalucía (P11-CTS-7730)es
dc.formatapplication/pdfes
dc.format.extent11 p.es
dc.language.isoenges
dc.publisherFrontiers Mediaes
dc.relation.ispartofFrontiers in Microbiology, 8, 1370.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCiprofloxacines
dc.subjectLow-level quinolone resistancees
dc.subjectSurvivales
dc.subjectTranscriptomices
dc.subjectGlobal responsees
dc.subjectSensitizationes
dc.titleCellular Response to Ciprofloxacin in Low-Level Quinolone-Resistant Escherichia colies
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.relation.projectIDPI11-00934es
dc.relation.projectIDPI14/00940es
dc.relation.projectIDP11-CTS-7730es
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2017.01370es
dc.identifier.doi10.3389/fmicb.2017.01370es
dc.journaltitleFrontiers in Microbiologyes
dc.publication.volumen8es
dc.publication.initialPage1370es
dc.contributor.funderMinisterio de Sanidad y Consumo. Españaes
dc.contributor.funderJunta de Andalucíaes

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