dc.creator | Machuca, Jesús | es |
dc.creator | Recacha, Esther | es |
dc.creator | Briales Martín, Alejandra | es |
dc.creator | Díaz de Alba, Paula | es |
dc.creator | Blázquez, Jesús | es |
dc.creator | Pascual Hernández, Álvaro | es |
dc.creator | Rodríguez Martínez, José Manuel | es |
dc.date.accessioned | 2020-05-06T06:25:00Z | |
dc.date.available | 2020-05-06T06:25:00Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Machuca, J., Recacha, E., Briales Martín, A., Díaz de Alba, P., Blázquez, J., Pascual, Á. y Rodríguez Martínez, J.M. (2017). Cellular Response to Ciprofloxacin in Low-Level Quinolone-Resistant Escherichia coli. Frontiers in Microbiology, 8, 1370. | |
dc.identifier.issn | 1664-302X | es |
dc.identifier.uri | https://hdl.handle.net/11441/96177 | |
dc.description.abstract | Bactericidal activity of quinolones has been related to a combination of DNA
fragmentation, reactive oxygen species (ROS) production and programmed cell death
(PCD) systems. The underlying molecular systems responsible for reducing bactericidal
effect during antimicrobial therapy in low-level quinolone resistance (LLQR) phenotypes
need to be clarified. To do this and also define possible new antimicrobial targets,
the transcriptome profile of isogenic Escherichia coli harboring quinolone resistance
mechanisms in the presence of a clinical relevant concentration of ciprofloxacin was
evaluated. A marked differential response to ciprofloxacin of either up- or downregulation
was observed in LLQR strains. Multiple genes implicated in ROS modulation (related to
the TCA cycle, aerobic respiration and detoxification systems) were upregulated (sdhC
up to 63.5-fold) in mutants with LLQR. SOS system components were downregulated
(recA up to 30.7-fold). yihE, a protective kinase coding for PCD, was also upregulated
(up to 5.2-fold). SdhC inhibition sensitized LLQR phenotypes (up to 1Log = 2.3 after
24 h). At clinically relevant concentrations of ciprofloxacin, gene expression patterns in
critical systems to bacterial survival and mutant development were significantly modified
in LLQR phenotypes. Chemical inhibition of SdhC (succinate dehydrogenase) validated
modulation of ROS as an interesting target for bacterial sensitization. | es |
dc.description.sponsorship | Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III ( PI11-00934, PI14/00940) | es |
dc.description.sponsorship | Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía (P11-CTS-7730) | es |
dc.format | application/pdf | es |
dc.format.extent | 11 p. | es |
dc.language.iso | eng | es |
dc.publisher | Frontiers Media | es |
dc.relation.ispartof | Frontiers in Microbiology, 8, 1370. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Ciprofloxacin | es |
dc.subject | Low-level quinolone resistance | es |
dc.subject | Survival | es |
dc.subject | Transcriptomic | es |
dc.subject | Global response | es |
dc.subject | Sensitization | es |
dc.title | Cellular Response to Ciprofloxacin in Low-Level Quinolone-Resistant Escherichia coli | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.projectID | PI11-00934 | es |
dc.relation.projectID | PI14/00940 | es |
dc.relation.projectID | P11-CTS-7730 | es |
dc.relation.publisherversion | https://doi.org/10.3389/fmicb.2017.01370 | es |
dc.identifier.doi | 10.3389/fmicb.2017.01370 | es |
dc.journaltitle | Frontiers in Microbiology | es |
dc.publication.volumen | 8 | es |
dc.publication.initialPage | 1370 | es |
dc.contributor.funder | Ministerio de Sanidad y Consumo. España | es |
dc.contributor.funder | Junta de Andalucía | es |