dc.creator | Ferrero, María Isabel | es |
dc.creator | Cano Rodríguez, María Mercedes | es |
dc.creator | Ilundáin Larrañeta, María Anunciación Ana | es |
dc.date.accessioned | 2020-04-24T15:55:06Z | |
dc.date.available | 2020-04-24T15:55:06Z | |
dc.date.issued | 1995 | |
dc.identifier.citation | Ferrero, M.I., Cano Rodríguez, M.M. y Ilundáin Larrañeta, M.A.A. (1995). Na+-H+ exchange activity in brush-border membrane vesicles isolated from chick small intestine. Biochimica et Biophysica Acta - Biomembranes, 1235 (2), 313-316. | |
dc.identifier.issn | 0005-2736 | es |
dc.identifier.issn | 1879-2642 | es |
dc.identifier.uri | https://hdl.handle.net/11441/95725 | |
dc.description.abstract | This study was undertaken to investigate the presence of a Na+{single bond}H+ antiporter in brush-border membrane vesicles (BBMV) isolated from chick small intestine. An outwardly directed proton gradient (pH 5.5 inside, 7.5 outside) stimulated Na+ uptake into BBMV and resulted in a transient accumulation. No accumulation was observed in the absence of a proton gradient. Voltage clamping the membrane with K+ and valinomycin decreased the Na+ overshoot. Amiloride inhibited pH gradient-driven Na+ uptake in a dose-dependent manner with an IC50 of 44 μM. The relationship between pH gradient-driven Na+ uptake and external Na+ concentration followed simple, saturating Michaelis-Menten kinetics. Eadie-Hofstee analysis of the pH gradient-driven Na+ uptake indicated a single transport system with a Vmax of 33 nmol/mg protein per 15 s and a Km for Na+ of 12 mM. The initial rate of pH-driven Na+ uptake increased as the intravesicular pH decreased, with a Hill coefficient close to 1. These findings indicate that BBMV isolated from chicken small intestine posses a Na+{single bond}H+ exchanger. This exchanger does not appear to be the one involved in cell pH regulation. | es |
dc.description.sponsorship | Dirección General de Investigaciones Científicas y Técnicas PB89-0616 | es |
dc.format | application/pdf | es |
dc.format.extent | 4 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Biochimica et Biophysica Acta - Biomembranes, 1235 (2), 313-316. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | BBMV | es |
dc.subject | Kinetics | es |
dc.subject | Sodium ion-proton exchange | es |
dc.title | Na+-H+ exchange activity in brush-border membrane vesicles isolated from chick small intestine | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología | es |
dc.relation.projectID | PB89-0616 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1016/0005-2736(95)80019-C | es |
dc.identifier.doi | 10.1016/0005-2736(95)80019-C | es |
dc.journaltitle | Biochimica et Biophysica Acta - Biomembranes | es |
dc.publication.volumen | 1235 | es |
dc.publication.issue | 2 | es |
dc.publication.initialPage | 313 | es |
dc.publication.endPage | 316 | es |