dc.creator | Horra Padilla, Carmen de la | es |
dc.creator | Cano Rodríguez, María Mercedes | es |
dc.creator | Peral Rubio, María José | es |
dc.creator | García Delgado, Marta | es |
dc.creator | Durán Martínez, Juan Manuel | es |
dc.creator | Calonge Castrillo, María Luisa | es |
dc.creator | Ilundáin Larrañeta, María Anunciación Ana | es |
dc.date.accessioned | 2020-04-24T15:42:35Z | |
dc.date.available | 2020-04-24T15:42:35Z | |
dc.date.issued | 2001 | |
dc.identifier.citation | Horra Padilla, C.d.l., Cano Rodríguez, M.M., Peral Rubio, M.J., García Delgado, M., Durán Martínez, J.M., Calonge Castrillo, M.L. y Ilundáin Larrañeta, M.A.A. (2001). Na+-dependent D-mannose transport at the apical membrane of rat small intestine and kidney cortex. Biochimica et Biophysica Acta - Biomembranes, 1512 (2), 225-230. | |
dc.identifier.issn | 0005-2736 | es |
dc.identifier.issn | 1879-2642 | es |
dc.identifier.uri | https://hdl.handle.net/11441/95724 | |
dc.description.abstract | The presence of a Na+/D-mannose cotransport activity in brush-border membrane vesicles (BBMV), isolated from either rat small intestine or rat kidney cortex, is examined. In the presence of an electrochemical Na+ gradient, but not in its absence, D-mannose was transiently accumulated by the BBMV. D-Mannose uptake into the BBMV was energized by both the electrical membrane potential and the Na+ chemical gradient. D-Mannose transport vs. external D-mannose concentration can be described by an equation that represents a superposition of a saturable component and another component that cannot be saturated up to 50 μM D-mannose. D-Mannose uptake was inhibited by D-mannose ≫ D-glucose > phlorizin, whereas for α-methyl glucopyranoside the order was D-glucose = phlorizin ≫ D-mannose. The initial rate of D-mannose uptake increased as the extravesicular Na+ concentration increased, with a Hill coefficient of 1, suggesting that the Na+ :D-mannose cotransport stoichiometry is 1:1. It is concluded that both rat intestinal and renal apical membrane have a concentrative, saturable, electrogenic and Na+-dependent D-mannose transport mechanism, which is different from SGLT1. | es |
dc.description.sponsorship | Dirección General de Investigaciones Científicas y Técnicas PM99-0121 | es |
dc.format | application/pdf | es |
dc.format.extent | 6 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Biochimica et Biophysica Acta - Biomembranes, 1512 (2), 225-230. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Brush-border membrane vesicle | es |
dc.subject | Intestine | es |
dc.subject | Kidney | es |
dc.subject | Sodium/D-mannose | es |
dc.title | Na+-dependent D-mannose transport at the apical membrane of rat small intestine and kidney cortex | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología | es |
dc.relation.projectID | PM99-0121 | es |
dc.relation.publisherversion | 10.1016/S0005-2736(01)00322-4 | es |
dc.identifier.doi | http://dx.doi.org/10.1016/S0005-2736(01)00322-4 | es |
dc.journaltitle | Biochimica et Biophysica Acta - Biomembranes | es |
dc.publication.volumen | 1512 | es |
dc.publication.issue | 2 | es |
dc.publication.initialPage | 225 | es |
dc.publication.endPage | 230 | es |