Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans

Mata Cabana, Alejandro; Sin, Olga; Seinstra, Renée I.; Nollen, Ellen A. A.

doi:10.21769/BioProtoc.3053

Artículo

dc.creator	Mata Cabana, Alejandro	es
dc.creator	Sin, Olga	es
dc.creator	Seinstra, Renée I.	es
dc.creator	Nollen, Ellen A. A.	es
dc.date.accessioned	2020-03-25T10:42:03Z
dc.date.available	2020-03-25T10:42:03Z
dc.date.issued	2018
dc.identifier.citation	Mata Cabana, A., Sin, O., Seinstra, R.I. y Nollen, E.A.A. (2018). Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans. Bio-protocol, 8 (20), e3053.
dc.identifier.issn	2331-8325 (electrónico)	es
dc.identifier.uri	https://hdl.handle.net/11441/94500
dc.description.abstract	C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans lysates, which can give insight into the location, function and/or toxicity of proteins	es
dc.description.sponsorship	National Institutes of Health National Centre for Research Resources (NIH)	es
dc.description.sponsorship	European Research Council (ERC)	es
dc.description.sponsorship	USANIH National Center for Research Resources (NCRR)	es
dc.description.sponsorship	Japan National BioResource Project	es
dc.format	application/pdf	es
dc.format.extent	15 p.	es
dc.language.iso	eng	es
dc.publisher	Bio-Protocol	es
dc.relation.ispartof	Bio-protocol, 8 (20), e3053.
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	C. elegans	es
dc.subject	Subcellular fractionation	es
dc.subject	Protein localization	es
dc.subject	Nucleus	es
dc.subject	Cytoplasm	es
dc.subject	Immunoblot	es
dc.title	Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans	es
dc.type	info:eu-repo/semantics/article	es
dcterms.identifier	https://ror.org/03yxnpp24
dc.type.version	info:eu-repo/semantics/publishedVersion	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	es
dc.relation.publisherversion	http://dx.doi.org/10.21769/BioProtoc.3053	es
dc.identifier.doi	10.21769/BioProtoc.3053	es
dc.journaltitle	Bio-protocol	es
dc.publication.volumen	8	es
dc.publication.issue	20	es
dc.publication.initialPage	e3053	es
dc.contributor.funder	National Institutes of Health. United States	es
dc.contributor.funder	European Research Council (ERC)	es
dc.contributor.funder	National Center for Research Resources (NCRR)	es

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