Article
Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans
Author/s | Mata Cabana, Alejandro
![]() ![]() ![]() ![]() ![]() ![]() ![]() Sin, Olga Seinstra, Renée I. Nollen, Ellen A. A. |
Date | 2018 |
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Abstract | C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole ... C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans lysates, which can give insight into the location, function and/or toxicity of proteins |
Funding agencies | National Institutes of Health. United States European Research Council (ERC) National Center for Research Resources (NCRR) |
Citation | Mata Cabana, A., Sin, O., Seinstra, R.I. y Nollen, E.A.A. (2018). Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans. Bio-protocol, 8 (20), e3053. |
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