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dc.creatorPérez Montaño, Francisco de Asíses
dc.creatorYang, Rongzhies
dc.creatorSantos García, Diegoes
dc.creatorMateus da Silva, Gustavoes
dc.creatorZhao, Meies
dc.creatorRosenberg, Tallyes
dc.creatorChen, Gonges
dc.date.accessioned2019-07-09T10:31:25Z
dc.date.available2019-07-09T10:31:25Z
dc.date.issued2019
dc.identifier.citationPérez Montaño, F.d.A., Yang, R., Santos García, D., Mateus da Silva, G., Zhao, M., Rosenberg, T. y Chen, G. (2019). Complete Assembly of the Genome of an Acidovorax citrulli Strain Reveals a Naturally Occurring Plasmid in This Species. Frontiers in Microbiology, 10 (art. 1400), 1-17.
dc.identifier.issn1664-302Xes
dc.identifier.urihttps://hdl.handle.net/11441/87953
dc.description.abstractAcidovorax citrulli is the causal agent of bacterial fruit blotch (BFB), a serious threat to cucurbit crop production worldwide. Based on genetic and phenotypic properties, A. citrulli strains are divided into two major groups: group I strains have been generally isolated from melon and other non-watermelon cucurbits, while group II strains are closely associated with watermelon. In a previous study, we reported the genome of the group I model strain, M6. At that time, the M6 genome was sequenced by MiSeq Illumina technology, with reads assembled into 139 contigs. Here, we report the assembly of the M6 genome following sequencing with PacBio technology. This approach not only allowed full assembly of the M6 genome, but it also revealed the occurrence of a ∼53 kb plasmid. The M6 plasmid, named pACM6, was further confirmed by plasmid extraction, Southern-blot analysis of restricted fragments and obtention of M6-derivative cured strains. pACM6 occurs at low copy numbers (average of ∼4.1 ± 1.3 chromosome equivalents) in A. citrulli M6 and contains 63 open reading frames (ORFs), most of which (55.6%) encoding hypothetical proteins. The plasmid contains several genes encoding type IV secretion components, and typical plasmid-borne genes involved in plasmid maintenance, replication and transfer. The plasmid also carries an operon encoding homologs of a Fic-VbhA toxin-antitoxin (TA) module. Transcriptome data from A. citrulli M6 revealed that, under the tested conditions, the genes encoding the components of this TA system are among the highest expressed genes in pACM6. Whether this TA module plays a role in pACM6 maintenance is still to be determined. Leaf infiltration and seed transmission assays revealed that, under tested conditions, the loss of pACM6 did not affect the virulence of A. citrulli M6. We also show that pACM6 or similar plasmids are present in several group I strains, but absent in all tested group II strains of A. citrulli.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherFrontiers Mediaes
dc.relation.ispartofFrontiers in Microbiology, 10 (art. 1400), 1-17.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAcidovorax citrullies
dc.subjectbacterial fruit blotches
dc.subjectSMRT sequencinges
dc.subjectplasmides
dc.titleComplete Assembly of the Genome of an Acidovorax citrulli Strain Reveals a Naturally Occurring Plasmid in This Specieses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.relation.publisherversionhttp://dx.doi.org/10.3389/fmicb.2019.01400es
dc.identifier.doi10.3389/fmicb.2019.01400es
idus.format.extent17 p.es
dc.journaltitleFrontiers in Microbiologyes
dc.publication.volumen10es
dc.publication.issueart. 1400es
dc.publication.initialPage1es
dc.publication.endPage17es

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