Show simple item record

dc.creatorSáez, Tamaraes
dc.creatorVos, Paul dees
dc.creatorKuipers, Jeroenes
dc.creatorSobrevia Luarte, Luises
dc.creatorFaas, Marijke M.es
dc.date.accessioned2019-04-26T09:50:35Z
dc.date.available2019-04-26T09:50:35Z
dc.date.issued2019
dc.identifier.citationSáez, T., Vos, P.d., Kuipers, J., Sobrevia Luarte, L. y Faas, M.M. (2019). Exosomes derived from monocytes and from endothelial cells mediate monocyte and endothelial cell activation under high d-glucose conditions. Immunology, 224 (2), 325-333.
dc.identifier.issn1365-2567es
dc.identifier.urihttps://hdl.handle.net/11441/86011
dc.description.abstractDiabetes mellitus type 2 (DMT2) is characterized by hyperglycemia and associated with low grade inflammation affecting both endothelial cells and monocytes. Exosomes are nanovesicles, allow communication between endothelial cells and monocytes and have been associated with diabetic complications. In this study we evaluated whether high glucose can activate monocytes and endothelial cells and whether exosomes play a role in this activation. Moreover, we studied whether endothelial cells and monocytes communicate with each other via exosomes under high and basal glncubation. In the first experiment, monomac 6 cells (MM6) were exposed to high glucose (HG; 25 mmol/L) or to exosomes from MM6 exposed to HG (exoMM6-HG) or basal glucose (5.5 mmol/L) (exoMM6-BG). In the second experiment, MM6 were exposed to exosomes from human umbilical vein endothelial cells (HUVECs) and HUVECs to exosomes from MM6. In the third experiment, MM6 and HUVECs were exposed to a mixture of exosomes from MM6 and HUVECs (exoMix). Cell activation was evaluated by measuring the protein surface expression of intracellular adhesion molecule-1 (ICAM-1) by flow cytometry. HG increased ICAM-1 expression in MM6 and monocytic exosomes from HG or BG shown similar effect in HG and BG MM6 cells. Exosomes from HUVECs increased ICAM-1 expression in MM6 cells, incubated under HG or BG, while also exosomes from MM6 increased ICAM-1 expression in HUVECs incubated under HG or BG. The combination of exosomes from both cell types (exoMixHG or exoMixBG) also increased ICAM-1 expression in both type cells in most conditions. However, the exoMixBG reversed the effect of HG in both MM6 and HUVECs. Our results show that HG activated monocytes and endothelial cells and that exosomes play a role in this HG-induced cell ICAM-1 expression. We hypothesize that during DMT2, exosomes may act as a communication mechanism between monocytes and endothelial cells, inducing and maintaining activating of both cell types in the presence of high glucose.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.relation.ispartofImmunology, 224 (2), 325-333.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectDiabetes mellitus type 2es
dc.subjectEndothelial cellses
dc.subjectExosomeses
dc.subjectHyperglycemiaes
dc.subjectICAM-1es
dc.subjectMonocyteses
dc.titleExosomes derived from monocytes and from endothelial cells mediate monocyte and endothelial cell activation under high d-glucose conditionses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Fisiologíaes
dc.relation.publisherversionhttps://doi.org/10.1016/j.imbio.2019.02.004es
dc.identifier.doi10.1016/j.imbio.2019.02.004es
idus.format.extent8 p.es
dc.journaltitleImmunologyes
dc.publication.volumen224es
dc.publication.issue2es
dc.publication.initialPage325es
dc.publication.endPage333es

FilesSizeFormatViewDescription
PUB1-s2.0-S0171298519300403-ma ...1.538MbIcon   [PDF] View/Open  

This item appears in the following collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional