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dc.creatorBernal Ibáñez, Maríaes
dc.creatorRoncel Gil, Mercedeses
dc.creatorOrtega Rodríguez, José Maríaes
dc.creatorPicorel Castaño, Rafaeles
dc.creatorYruela Guerrero, Inmaculadaes
dc.date.accessioned2019-04-02T11:32:17Z
dc.date.available2019-04-02T11:32:17Z
dc.date.issued2004
dc.identifier.citationBernal Ibáñez, M., Roncel Gil, M., Ortega Rodríguez, J.M., Picorel Castaño, R. y Yruela Guerrero, I. (2004). Copper effect on cytochrome b559 of photosystem II under photoinhibitory conditions. Physiologia Plantarum, 120 (4), 686-694.
dc.identifier.issn0031-9317 (impreso)es
dc.identifier.issn1399-3054 (electrónico)es
dc.identifier.urihttps://hdl.handle.net/11441/85059
dc.description.abstractToxic Cu (II) effect on cytochrome b559 under aerobic photoinhibitory conditions was examined in two different photosystem II (PSII) membrane preparations active in oxygen evolution. The preparations differ in the content of cytochrome b559 redox potential forms. Difference absorption spectra showed that the presence of Cu (II) induced the oxidation of the high‐potential form of cytochrome b559 in the dark. Addition of hydroquinone reduced the total oxidized high‐potential form of cytochrome b559 present in Cu (II)‐treated PSII membranes indicating that no conversion to the low‐potential form took place. Spectroscopic determinations of cytochrome b559 during photoinhibitory treatment showed slower kinetics of Cu (II) effect on cytochrome b559 in comparison with the rapid loss of oxygen evolution activity in the same conditions. This result indicates that cytochrome b559 is affected after PSII centres are photoinhibited. The high‐potential form was more sensitive to toxic Cu (II) action than the low‐potential form under illumination at pH 6.0. The content of the high‐potential form of cytochrome b559 was completely lost; however, the low‐potential content was unaffected in these conditions. This loss did not involve cytochrome protein degradation. The results are discussed in terms of different binding properties of the heme iron to the protonated or unprotonated histidine ligand in the high‐potential and low‐potential forms of cytochrome b559, respectively.es
dc.description.sponsorshipDirección General de Investigación BMC2002-00031es
dc.description.sponsorshipGobierno de Aragón P015/2001es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherWileyes
dc.relation.ispartofPhysiologia Plantarum, 120 (4), 686-694.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCytochrome b559es
dc.subjectCopperes
dc.subjectPhotosystem IIes
dc.subjectPhotoinhibitiones
dc.subjectRedox potentiales
dc.titleCopper effect on cytochrome b559 of photosystem II under photoinhibitory conditionses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/acceptedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectIDBMC2002-00031es
dc.relation.projectIDP015/2001es
dc.relation.publisherversionhttps://doi.org/10.1111/j.1399-3054.2004.0286.xes
dc.identifier.doi10.1111/j.1399-3054.2004.0286.xes
idus.format.extent9 p.es
dc.journaltitlePhysiologia Plantarumes
dc.publication.volumen120es
dc.publication.issue4es
dc.publication.initialPage686es
dc.publication.endPage694es

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