dc.creator | Yruela Guerrero, Inmaculada | es |
dc.creator | Torrado, Elena | es |
dc.creator | Roncel Gil, Mercedes | es |
dc.creator | Picorel Castaño, Rafael | es |
dc.date.accessioned | 2019-03-29T13:11:45Z | |
dc.date.available | 2019-03-29T13:11:45Z | |
dc.date.issued | 2001 | |
dc.identifier.citation | Yruela Guerrero, I., Torrado, E., Roncel Gil, M. y Picorel Castaño, R. (2001). Light-induced absorption spectra of the D1–D2–cytochrome b 559 complex of Photosystem II: Effect of methyl viologen concentration. Photosynthesis Research, 67 (3), 199-206. | |
dc.identifier.issn | 0166-8595 (impreso) | es |
dc.identifier.issn | 1573-5079 (electrónico) | es |
dc.identifier.uri | https://hdl.handle.net/11441/84951 | |
dc.description.abstract | The light-induced difference absorption spectra associated to the photo-accumulation of reduced pheophytin a were studied in the isolated D1–D2–Cyt b559 complex in the presence of variable methyl viologen concentrations and different illumination conditions under anaerobiosis. Depending on the methyl viologen/reaction centre ratio, the relative intensities of the spectral bands at 681.5±0.5, 667.0±0.5 and 542.5±0.5 nm were modified. The reduced pheophytin a located at the D1-branch of the complex absorbs at 681.7±0.5 nm, and at least two additional pigment species contribute to the Qy band of the difference absorption spectra with maxima at 667.0±0.5 and 680.5±0.5 nm. We propose the additional species correspond to a peripheral chlorophyll a and the pheophytin a located at the D2-branch of the complex, respectively. The blue absorbing chlorophyll at 667 nm is susceptible to chemical redox changes with a midpoint reduction potential of +470 mV. The Qx absorption bands of both pheophytins localised at the D2- and D1-branch of the D1–D2–Cyt b559 complex were at 540.7±0.5 and 542.9±0.5, respectively. The results indicated that the two pheophytin molecules can be photoreduced in the D1–D2–Cyt b559 complex in certain experimental conditions. | es |
dc.description.sponsorship | Dirección General de Investigación Científica y Técnica PB98-1632 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Springer Verlag | es |
dc.relation.ispartof | Photosynthesis Research, 67 (3), 199-206. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Absorption | es |
dc.subject | Chlorophyll | es |
dc.subject | Pheophytin | es |
dc.subject | Photosystem II | es |
dc.subject | Reaction centre | es |
dc.subject | Spectroscopy | es |
dc.title | Light-induced absorption spectra of the D1–D2–cytochrome b 559 complex of Photosystem II: Effect of methyl viologen concentration | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | PB98-1632 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1023/A:1010682416016 | es |
dc.identifier.doi | 10.1023/A:1010682416016 | es |
idus.format.extent | 8 p. | es |
dc.journaltitle | Photosynthesis Research | es |
dc.publication.volumen | 67 | es |
dc.publication.issue | 3 | es |
dc.publication.initialPage | 199 | es |
dc.publication.endPage | 206 | es |