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Artículo
NsrR1, a Nitrogen stress-repressed sRNA, contributes to the regulation of nblA in Nostoc sp. PCC 7120
dc.creator | Álvarez Escribano, Isidro | es |
dc.creator | Vioque Peña, Agustín | es |
dc.creator | Muro Pastor, Alicia María | es |
dc.date.accessioned | 2019-03-21T10:56:42Z | |
dc.date.available | 2019-03-21T10:56:42Z | |
dc.date.issued | 2018-09-24 | |
dc.identifier.citation | Álvarez Escribano, I., Vioque Peña, A. y Muro Pastor, A.M. (2018). NsrR1, a Nitrogen stress-repressed sRNA, contributes to the regulation of nblA in Nostoc sp. PCC 7120. Frontiers in Microbiology, 9 (2267), 1-14. | |
dc.identifier.issn | 1664-302X | es |
dc.identifier.uri | https://hdl.handle.net/11441/84511 | |
dc.description.abstract | Small regulatory RNAs (sRNAs) are currently considered as major post-transcriptional regulators of gene expression in bacteria. The interplay between sRNAs and transcription factors leads to complex regulatory networks in which both transcription factors and sRNAs may appear as nodes. In cyanobacteria, the responses to nitrogen availability are controlled at the transcriptional level by NtcA, a CRP/FNR family regulator. In this study, we describe an NtcA-regulated sRNA in the cyanobacterium Nostoc sp. PCC 7120, that we have named NsrR1 (nitrogen stress repressed RNA1). We show sequence specific binding of NtcA to the promoter of NsrR1. Prediction of possible mRNA targets regulated by NsrR1 allowed the identification of nblA, encoding a protein adaptor for phycobilisome degradation under several stress conditions, including nitrogen deficiency. We demonstrate specific interaction between NsrR1 and the 5′-UTR of the nblA mRNA, that leads to decreased expression of nblA. Because both NsrR1 and NblA are under transcriptional control of NtcA, this regulatory circuit constitutes a coherent feed-forward loop, involving a transcription factor and an sRNA. | es |
dc.description.sponsorship | Agencia Estatal de Investigación (AEI) BFU2016-74943- C2-1-P | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad BFU2013-48282-C2-1-P, BES-2014- 068488 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Frontiers Media | es |
dc.relation.ispartof | Frontiers in Microbiology, 9 (2267), 1-14. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | NtcA | es |
dc.subject | Regulatory RNA | es |
dc.subject | Cyanobacteria | es |
dc.subject | Feed-forward loop | es |
dc.subject | Post-transcriptional regulation | es |
dc.title | NsrR1, a Nitrogen stress-repressed sRNA, contributes to the regulation of nblA in Nostoc sp. PCC 7120 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | BFU2013-48282-C2-1-P | es |
dc.relation.projectID | BFU2016-74943- C2-1-P | es |
dc.relation.projectID | BES-2014- 068488 | es |
dc.relation.publisherversion | https://doi.org/10.3389/fmicb.2018.02267 | es |
dc.identifier.doi | 10.3389/fmicb.2018.02267 | es |
idus.format.extent | 14 p. | es |
dc.journaltitle | Frontiers in Microbiology | es |
dc.publication.volumen | 9 | es |
dc.publication.issue | 2267 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 14 | es |