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dc.creatorSzydlowski, Nicolases
dc.creatorRagel de la Torre, Paulaes
dc.creatorHennen-Bierwagen, Tracie A.es
dc.creatorPlanchot, Veroniquees
dc.creatorMyers, Alan M.es
dc.creatorMérida Berlanga, Ángeles
dc.creatorD'Hulst, Christophees
dc.creatorWattebled, Fabricees
dc.date.accessioned2019-03-21T09:47:22Z
dc.date.available2019-03-21T09:47:22Z
dc.date.issued2011
dc.identifier.citationSzydlowski, N., Ragel de la Torre, P., Hennen-Bierwagen, T.A., Planchot, V., Myers, A.M., Mérida Berlanga, Á.,...,Wattebled, F. (2011). Integrated functions among multiple starch synthases determine both amylopectin chain length and branch linkage location in Arabidopsis leaf starch. Journal of Experimental Botany, 62 (13), 4547-4559.
dc.identifier.issn0022-0957es
dc.identifier.urihttps://hdl.handle.net/11441/84505
dc.description.abstractThis study assessed the impact on starch metabolism in Arabidopsis leaves of simultaneously eliminating multiple soluble starch synthases (SS) from among SS1, SS2, and SS3. Double mutant ss1- ss2- or ss1- ss3- lines were generated using confirmed null mutations. These were compared to the wild type, each single mutant, and ss1- ss2- ss3- triple mutant lines grown in standardized environments. Double mutant plants developed similarly to the wild type, although they accumulated less leaf starch in both short-day and long-day diurnal cycles. Despite the reduced levels in the double mutants, lines containing only SS2 and SS4, or SS3 and SS4, are able to produce substantial amounts of starch granules. In both double mutants the residual starch was structurally modified including higher ratios of amylose:amylopectin, altered glucan chain length distribution within amylopectin, abnormal granule morphology, and altered placement of α(1→6) branch linkages relative to the reducing end of each linear chain. The data demonstrate that SS activity affects not only chain elongation but also the net result of branch placement accomplished by the balanced activities of starch branching enzymes and starch debranching enzymes. SS3 was shown partially to overlap in function with SS1 for the generation of short glucan chains within amylopectin. Compensatory functions that, in some instances, allow continued residual starch production in the absence of specific SS classes were identified, probaby accomplished by the granule bound starch synthase GBSS1.es
dc.description.sponsorshipANR Génoplante GPLA0611Ges
dc.description.sponsorshipEuropean Union-FEDER, Région Nord Pas de Calais ARCir PlantTEQ5es
dc.description.sponsorshipNational Science Foundation DBI-0209789es
dc.description.sponsorshipComisión Interministerial de Ciencia y Tecnología BIO2009-07040es
dc.description.sponsorshipJunta de Andalucía P09-CVI-4704es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherOxford University Presses
dc.relation.ispartofJournal of Experimental Botany, 62 (13), 4547-4559.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAmylopectines
dc.subjectAmylosees
dc.subjectBranchinges
dc.subjectChain-length-distributiones
dc.subjectGlucanses
dc.subjectSS1es
dc.subjectSS2es
dc.subjectSS3es
dc.subjectStarches
dc.subjectStarch synthaseses
dc.titleIntegrated functions among multiple starch synthases determine both amylopectin chain length and branch linkage location in Arabidopsis leaf starches
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/acceptedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectIDGPLA0611Ges
dc.relation.projectIDARCir PlantTEQ5es
dc.relation.projectIDDBI-0209789es
dc.relation.projectIDBIO2009-07040es
dc.relation.projectIDP09-CVI-4704es
dc.relation.publisherversionhttps://doi.org/10.1093/jxb/err172es
dc.identifier.doi10.1093/jxb/err172es
idus.format.extent13 p.es
dc.journaltitleJournal of Experimental Botanyes
dc.publication.volumen62es
dc.publication.issue13es
dc.publication.initialPage4547es
dc.publication.endPage4559es

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